versión On-line ISSN 1025-0298
CAMACHO, Frank et al. In vitro gene transfer using cationic polymers. Vaccimonitor [online]. 2006, vol.15, n.1, pp. 17-21. ISSN 1025-0298.
Gene transfer represents an important tool for the study, prevention and treatment of genetic and acquired diseases, as well as for protein function studies. Because of their efficiency, the use of cationic molecules has become a strong competitor to other gene transfer methods. In this study, COS-7 and BHK-21 cell lines were transfected with the plasmid psnEGFP using the cationic polymers polyethylene imine (PEI) and DEAE-dextran. When transfection efficiency was measured, it was observed that the cell line BHK-21 is an excellent option for transfection with the PEI method. The best results were obtained when PEI was dissolved in NaCl 150 mM. When plates were centrifuged after addition of the PEI-DNA complexes, nearly 100% of transfected cells with low DNA concentrations were obtained.
Palabras llave : Transfection; polyethylene imine; PEI; gene transfer; cationic polymers.