Abstract

OROPESA, Suset et al. Production of a specific antiserum against strain A/Johannesburg/33/94 (H3N2), and its usefulness in characterizing isolations of Influenza virus in Wistar rats. Vaccimonitor [online]. 2006, vol.15, n.3, pp.9-13. ISSN 1025-028X.

A polyclonal antiserum with high titer, great specificity and sensitivity was obtained against the hemagglutinin of Influenza A/Johannesburg/33/94, belonging to subtype A (H3N2). The obtaining process of the antigen or immunogen, the immunization schedule and its administration to Wistar rats as animal model are described. All of these were validated by the hemagglutinin inhibition technique. The antiserum obtained was treated with receptor-destroying enzymes of national production (EDR/IPK) in order to eliminate the unspecific inhibitors of the hemagglutinin inhibition. Validation was carried out in front of a panel including 9 influenza reference strains belonging to subtypes A(H3N2), A(H1N1), and to type B. No unspecific reactions against strains A(H1N1) and of type B were detected. Five autochthonous isolates were satisfactorily typed and subtyped.

Keywords : Influenza virus; polyclonal antisera; antigenic characterization; hemagglutination inhibition..

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