Abstract

PEREZ-REYTOR, Diliana Celeste; DOMINGUEZ-VAZQUEZ, Iveldris; OLANO-RUIZ, Evelyn  and  SOSA-ESPINOSA, Angela Estela. Strategy to verify quality of Escherichia coli strains kept in the collection of the Center of Genetic Engineering and Biotechnology. Vaccimonitor [online]. 2010, vol.19, n.1, pp.9-15. ISSN 1025-028X.

The Collection of Biotechnological-Interest Microorganisms of the CIGB includes several bacteria and yeast strains used in gene manipulation by means of genetic engineering and the production of recombinant proteins. The E. coli host collection contains all the strains used in the research projects of the institution. This microorganism is an important tool for genetic engineering and molecular research. Accuracy of results depends on the quality of the microorganism banks, which it is determined by the storage conditions and the evaluation strategies of these microorganisms. The complexity of the management of this collection is influenced by the fact that a great number of contaminants may grow in the propagation media and they are difficult to identify. The more similar is the pollutant to the strain of interest, the harder it is to obtain pure banks with reliable stability and conservation. This work describes how quality control is ensured in the E. coli banks. The verification methods and strategies described here have been developed in our laboratory.

Keywords : Culture collection; E. coli strains; purity assay; Genetic Engineering; Molecular Research.

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