SciELO - Scientific Electronic Library Online

 
vol.21 número1Protección inducida por nanococleatos derivados de proteoliposomas de Leptospira interrogans serovar CanicolaBiodistribución de IgA secretora purificada de calostro humano en fluidos biológicos de ratón Balb/c índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Revista

Articulo

Indicadores

  • No hay articulos citadosCitado por SciELO

Links relacionados

  • No hay articulos similaresSimilares en SciELO

Compartir


Vaccimonitor

versión impresa ISSN 1025-028X

Resumen

BURGUET, Nancy  y  SIERRA, Nelson. Assessment of a preservation strategy for Brevundimonas diminuta. Vaccimonitor [online]. 2012, vol.21, n.1, pp.10-13. ISSN 1025-028X.

Brevundimonas diminuta is internationally recognized as the standard micro-organism for bacterial challenge tests. The Quality Control Laboratory of Liorad Laboratories has a collection of reference strains, among them Brevundimonas diminuta are kept by the freezing method at -70oC, replicated in Lactose Broth Saline (CLS) culture medium. This methodology was not the most convenient, that is why another strategy was assessed. Triptone Soy Broth was used as growth method, skim milk 20% as protective substance and lyophilization as preservation method. An adequate quality control, which included purity verification, viability and stability of the properties, was carried out. Results obtained during the 24 months of the stability study in real time, suggest that the chosen method would provide an alternative and solution to the problem of this strain preservation.

Palabras clave : Brevundimonas diminuta; stability; protective substance; liophilization.

        · resumen en Español     · texto en Español     · Español ( pdf )

 

Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons