Abstract

SANCHEZ-ENAMORADO, Yenner et al. Molecular characterization of Pasteurella multocida strains from cattle. Vaccimonitor [online]. 2015, vol.24, n.2. ISSN 1025-028X.

Pasteurellosis is a disease caused by Pasteurella multocida bacterium, which affects a variety of domestic and wild animals. P. multocida is a Gram negative, non-motile, and facultative anaerobic cocobacillus, from genus Pasteurella. Genotyping methods are useful to improve the limitations of the traditional phenotyping methods. The aim of this paper was to determine the genetic relationship between P. multocida strains B1, B2 and B3, proposed as vaccine candidates. The strains came from the culture collection of the Viral and Bacterial Vaccine Producing Enterprise, LABIOFAM (acronym in Spanish), and were analyzed with polymerase chain reaction and pulsed field electrophoresis. It was confirmed that the three strains belonged to P. multocida species, capsular type A multocida subspecies. It was also proved that B1 and B3 strains are genetically indistinguishable, and that B2 had a 91% of similarity with B1 and B3. Results obtained by the identification and classification of these strains will permit the development of vaccines against P. multocida.

Keywords : Pasteurella multocida; PCR; pulsed field gel electrophoresis.

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