Abstract

CAMPOS-CUELLO, Tania; COROAS-GONZALEZ, Laura  and  MARTINEZ-RODRIGUEZ, Livany. Detection by real time RT-PCR of the vaccine virus in pigs immunized with the Cuban vaccine against classical swine fever. Vaccimonitor [online]. 2016, vol.25, n.2, pp.37-42. ISSN 1025-028X.

Classical swine fever (CSF) is a highly contagious viral disease caused by a RNA virus that belongs to the genus Pestivirus within the family Flaviviridae. CSF represents one of the leading worldwide threats to the pig industry. Life attenuated vaccines using lapinized Chinese strain have been used to prevent the disease. The reverse transcriptase polymerase chain reaction (RT-PCR) is one of the more sensitive methods that have been used in veterinary medicine for RNA virus detection. In the case of CSF virus is very useful, because the nucleic acid could be detected in early stages of the infection and during a long period in recovered animals. The aim of this study was to apply real time RT-PCR assay for the detection of lapinized Chinese strain from the Cuban vaccine strain against CSF. Tonsils of vaccinated pigs were the most positive organ in the detection of RNA vaccinal virus. Interference of the vaccinal virus in diagnosis was proved. The lower threshold cycle values were seen at 12 postvaccination day.

Keywords : classical swine fever; life attenuated vaccines; lapinized Chinese strain; RT-PCR.

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