Abstract

SEPULVEDA-ESTAY, Constanza Isabel; JARA-OSORIO, María Antonieta  and  NAVARRO-VENEGAS, Carlos Osvaldo. Molecular detection of glycoprotein B from feline herpesvirus. Vaccimonitor [online]. 2019, vol.28, n.3, pp.103-109.  Epub Dec 01, 2019. ISSN 1025-028X.

The feline herpesvirus type 1, generates multiple problems and the cat ends with consequences that affect its future quality of life. This virus is distributed throughout the world and is easily transmitted and since it is a latent pathogen, it continues to spread uncontrollably to the entire cat population. The diagnosis is based on clinical signs, today there is only a specific laboratory diagnostic method implemented in Chile to identify the agent, which is not used regularly in the clinic of small animals. Thus, the treatment and diagnosis are usually based on the knowledge and experience of veterinarian without leaving a real confirmation about which agent is causing the symptoms. This research proposed a molecular diagnostic method alternative to timidine kinase detection gene, for which cats under 1 year of age were selected, with symptoms compatible with an infection with the feline herpesvirus. The Polymerase Chain Reaction (PCR) method was used to detect the feline herpesvirus type 1 glycoprotein B gene, followed by the determination of the percentage of nucleotide identity (NIP) from official GenBank® data. Of the 11 cats studied, only one of them resulted in the amplification of a segment corresponding to the glycoprotein B gene. The resulting NIP (> 96%) confirms that the sequence obtained corresponds to type 1 glycoprotein B gene of feline herpesvirus and the efficiency of the method implemented is discussed.

Keywords : PCR; cat diseases; databases nucleic acid; feline herpesvirus type 1.

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