SciELO - Scientific Electronic Library Online

 
vol.29 número4Mejoras en el proceso evaluativo de la calidad del diagnóstico en los laboratorios con tecnología SUMAMejora genética de microtubérculos de papa in vitro mediante irradiación gamma índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Revista

Articulo

Indicadores

  • No hay articulos citadosCitado por SciELO

Links relacionados

  • No hay articulos similaresSimilares en SciELO

Compartir


Biotecnología Aplicada

versión On-line ISSN 1027-2852

Resumen

FERNANDEZ, Osmán; DUSTET, Julio C  y  CHICO, Ernesto. Mathematical model for the application of Metabolic Flux Analysis to CHO cells producing recombinant human erythropoietin. Biotecnol Apl [online]. 2012, vol.29, n.4, pp.246-252. ISSN 1027-2852.

Mathematical modeling of metabolism meets several important applications in the context of bioprocess engineering, such as the interpretation of cell physiology. Metabolic Flux Analysis, one of the tools of this discipline, was used in the present work to characterize the biosynthesis of recombinant human erythropoietin in CHO cells. In order to apply this method, we built a matrix of stoichiometric numbers representing the major metabolic pathways for the generation of energy and the synthesis of essential precursors for product and biomass accumulation. Equations representing the biosynthesis of recombinant human erythropoietin and the growth of CHO were also derived, conferring an advantage to the proposed model over other existing designs. The dimensions of the obtained matrix were 47 × 44, with a rank of 44 and a condition number of 83; therefore, the model has a unique solution and is not sensitive. The metabolic flux map obtained by solving the mathematical model using experimental data showed results consistent with the known biochemistry of CHO cells and with the findings of other reports on this and other mammalian cell lines. The general steps of the methodology used to obtain the proposed mathematical model are also outlined.

Palabras clave : metabolic flux analysis; metabolism; mathematical model; CHO; EPO.

        · resumen en Español     · texto en Inglés     · Inglés ( pdf )