Revista Cubana de Plantas Medicinales
versión On-line ISSN 1028-4796
MARTINEZ, María Julia et al. Estudio toxicológico preclínico de la Psidium guajava L. (guayaba). Rev Cubana Plant Med [online]. 2001, vol.6, n.2, pp. 56-61. ISSN 1028-4796.
This toxicologic study was conducted using dry leaves of Psidium guajava L. In this plant material we performed an acute toxicologic study by 2 methods: 1) mean lethal dose LD50 test in Swiss mice (OF1) and 2) alternative toxicology (acute toxic classes) in Wistar rats. We also made the genotoxic assesment of 2 extracts, one of aqueous type, and the other of henaxic type in an in vitro system of short-term somatic segregation induction assay in the Aspergillus nidulans fungus and an in vivo assay of the dry drug in mouse bone marrow micronuclei induction test. No deaths were observed in the toxocological results of the 2 experimental models in the dose range using up to 2 000 mg/kg/b.w. The histological results did not suggest any damage attributable to toxicity of the studied plant material. In the in vitro study with Aspergillus nidulans D-30, it was observed the lack of genotoxic effect of these extracts, as well as in the mouse bone marrow micronucelus induction system.
Palabras llave : PLANT EXTRACTS [toxicity]; PLANT EXTRACTS [adverse effects]; PLANTS, MEDICINAL; ANIMALS, LABORATORY; ASPERGILLUS NIDULANS; MICRONUCLEUS TESTS; MUTAGENICITY TESTS.