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Revista Cubana de Plantas Medicinales

versão On-line ISSN 1028-4796

Resumo

LUA FIGUEIREDO, Solange Faria et al. Rollinia mucosa (Jacq.) Baill.: establishment of callus culture and lignan production. Rev Cubana Plant Med [online]. 2003, vol.8, n.3. ISSN 1028-4796.

Callus cultures were established from different organs of seedlings and in vitro propagated plants of Rollinia mucosa. The growth rate, type of callus and furofuranic lignan biosynthetic pattern were significantly influenced by the origin of the plant material, the explant type and the growth regulators used: 2,4-dichlorophenoxyacetic acid, naphthaleneacetic acid, 6-benzyladenine, gibberellic acid, and picloram.The efficiency of callus production and lignan synthesis was significantly higher in foliar blade explants in the majority of media. The best biomass was obtained on media with picloram. In seedlings explants, naphthaleneacetic acid and 2,4-dichlorophenoxyacetic acid induced epiyangambin synthesis in calli from foliar blade and magnolin in calli from epicotyl and petiole. In in vitro propagated plants explants, the synthesis of epiyangambin was induced by picloram only in calli from stem. Calli from foliar blade cultured in 10,4, 20,8 and 31,2 µM of picloram presented a two-three fold increase in epiyangambin rates with regard to levels detected in the original plant.

Palavras-chave : ROLLINIA; CULTURE MEDIA.

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