Resumen

SANCHEZ LAMAR, Ángel et al. Punica granatum L. whole fruit extract as a protection against the hydrogen peroxide-induced damage. Rev Cubana Plant Med [online]. 2005, vol.10, n.2. ISSN 1028-4796.

Punica granatum L. (Punicaceae) is a medicinal plant whose fruit has therapeutical properties. The whole fruit extract is under preclinical study, with the objective of being used for viral disease treatment. In the last few years, some experimental research has been aimed at studying the antigenotoxic characteristics of this extract as an additional valuable property. Some phytocomponents of Punica granatum L. fruit show powerful antioxidative activity and certain experimental results prove their capacity as natural antimutagenic agents. The present paper set forth the results achieved in evaluating the capacity of this extract to reduce hydrogen peroxide-induced damage in in vitro cultured ovarian cells from Chinese Hamster. Cytogenetic experiments such as the exchange of sister chromatids and the cytofluometric test, i.e, oxidized diclofluorescein fluorimetry were performed. Under the experimental conditions of this research work, the Punica granatum whole fruit extract could sequestrate reactive oxygen species caused by hydrogen peroxide, a mechanism that allows it to protect the DNA against the lesions provoked by this agent.

Palabras clave : Punica granatum L.; Chinese Hamster ovarian cells; anti-genotoxicity; anti-mutagenicity; hydrogen peroxide; exchange of sister chromatides..

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