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Revista Cubana de Plantas Medicinales

versión On-line ISSN 1028-4796

Resumen

BARRESE PEREZ, Yinet; HERNANDEZ JIMENEZ, María Elena  y  GARCIA PULPEIRO, Oscar. Analytical technique to quantify the anthraquinones present in Senna alata (L.) Roxb. (Guacamaya francesa). Rev Cubana Plant Med [online]. 2005, vol.10, n.3-4. ISSN 1028-4796.

An analytical quantitative technique that allows to determine the concentration of quinones present in the crude drug and in the fluid extract of Senna alata (L.) Roxb., popularly known as “Guacamaya francesa”, is exposed. Some aspects of the analytical procedure, such as the reflux time, the color development time and the way in which the ethereal phases were worked with, were modified. 3 methods of quantification were studied. All of them showed a good lineality, exactness and precision, but the differential spectrophotometry method was selected since it reduced in a higher percentage the interferences due to the degradation products, and it also had the highest specificity and the lowest percentage of error. A reference solution of CoCl2 was used. The modifications introduced in the analytical procedure allowed to have a fast and simple technique to be applied in a pharmaceutical production center.

Palabras clave : Senna alata (L.) Roxb; crude drug; fluid extract; reflux; ethereal phases.

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