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Revista Cubana de Plantas Medicinales
versão On-line ISSN 1028-4796
Resumo
CESTY BORDA-YEPEZ, Charlotte; SAENZ TEJADA, Erik C e PEREIRA LIMA, Giuseppina Pace. Micropropagation of Pothomorphe umbellata (L.) Miq. via direct organogenesis. Rev Cubana Plant Med [online]. 2007, vol.12, n.4. ISSN 1028-4796.
The present research was aimed at standardizing the protocol of seed disinfection, seed germination and organogenesis via callus of Pothomorphe umbellata (L.) Miq.The germinated seeds were inoculated in different concentrations of BAP (benzylamine purine) and NAA (naphthalene acetic acid) in order to stimulate the callus induction. After 60 days of culture, the calluses with some shoots were taken to an organogenesis medium (GA3 0.1 mg.L-1, BAP 0.5 mg.L-1) during 40 days, to be transferred later to a development medium. Finally, the plantules were acclimatized, presenting a good index of survival.
Palavras-chave : Pothomorphe umbellata; growth regulators; medicinal plants.