Resumen

ROMERO DIAZ, Jacqueline Aylema et al. Validation of a High Performance Liquid Chromatography technique for determination of mangiferin content in Mangifera indica L. leaves. Rev Cubana Plant Med [online]. 2014, vol.19, n.3, pp.167-178. ISSN 1028-4796.

Introduction: Mangiferin is a natural glycosylxanthone present in various parts of the tree Mangifera indica L. which is attributed a wide range of pharmacological properties. Objective: establish a method to determine the content of mangiferin in Mangifera Indica L. leaves by high performance liquid chromatography. Methods: leaves were dried and pulverized before quantification of their metabolite content. Separation was performed using a Luna RP-18 chromatographic column (5 µm) (250 x 4mm) with a UV detector at 254n and a mobile phase A gradient composed of acetic acid 0.2 %: acetonitrile (85:15), and a phase B mobile: acetonitrile and its quantification vs. a reference sample applying the external standard method. Results: the regression curve equation for mangiferin was Y=3.5561X +6536.55(r=0.999). Recovery was 99.41 % with a variation coefficient below 2 %. Detection and quantification limits were 0.0009 and 0.001, respectively. No statistically significant differences were found between the determinations performed on different days by different analysts. Conclusion: the method is simple and reliable, and may be used for quality control and stability studies about Mangifera Indica L. leaves.

Palabras clave : mangiferin; HPLC; validation.

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