Abstract

ARGOTA PEREZ, George; IANNACONE OLIVER, José  and  EGUREN IRIARTE, Gabriela. Total proteins and bioconcentration factor by metal exposure in Gambusia punctata (Poeciliidae). MEDISAN [online]. 2012, vol.16, n.11, pp.1731-1735. ISSN 1029-3019.

The aim of this study was to evaluate levels of total proteins and the bioconcentration factor by metal exposure in Gambusia punctata. The species was sampled in the ecosystem Filé and then transferred to the laboratory, where 3 treatments in 2 replications with 25 copies were designed. Mean lethal concentration (CL-50) was determined as a toxicity parameter for 48 hours of bioassay. The analyzed metals were lead and cadmium, quantified by plasma spectroscopy inductively coupled with axial view. After the experiment, the CL-50 corresponded to 0.1 and concentrations of 0.06 and 5.78 mg/L and the negative control were tested. Then the level of total proteins and metals in water, tissue and its relationship by means of the bioconcentration factor were quantified. The lower value of proteins was by exposure to cadmium with 43.9 % of inhibition (p <0.05) compared with the control; for lead 2.5 % of stimulation was determined. The highest concentrations in water and tissue corresponded to the latter, but not for the bioconcentration factor. It was concluded that the results showed sensitivity in the response of total protein content and a high bioaccumulative capacity for both metals.

Keywords : total protein; bioconcentration; lead; cadmium; Gambusia punctata.

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