SciELO - Scientific Electronic Library Online

 
vol.25 issue3Use of the corneal topography for a better professional training in patients with pterigion surgerySpontaneous contractile dynamics of the vascular smooth muscle of the external carotid artery author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

  • Have no cited articlesCited by SciELO

Related links

  • Have no similar articlesSimilars in SciELO

Share


MEDISAN

On-line version ISSN 1029-3019

Abstract

CHACON MARTEN, Alejandro Vladimir. Evaluation of sheep blood as nutritive suplement in culture media: citratated and defibrinated. MEDISAN [online]. 2021, vol.25, n.3, pp.686-702.  Epub June 11, 2021. ISSN 1029-3019.

Introduction:

Sheep blood is an essential supplement in the elaboration of culture media, as it provides important nutritional factors for the growth and recovery of different organisms.

Objective:

To evaluate comparatively the effect of sheep citrated and defibrinated sheep blood in culture media with agar base as for the bacterial growth and the production of hemolysis in reference strains from different pathogen bacteria, as well as the recovery or isolation of microorganisms from clinical samples.

Method:

An observational, descriptive and cross-sectional was carried out in 6 microbiology laboratories in Santiago de Cuba city during 2017, in which male sheeps blood from the pelibuey breed for elaborating culture media. Each laboratory received either citrated blood or defibrinated and a survey was delivered to evaluate the results.

Results:

There was a better growing and bacterial isolation in the media supplemented with defibrinated blood, although yielding or results were favorable with citrated blood.

Conclusions:

The pertinence of the use of defibrinated blood as a supplement of nutritive enrichment in culture media was confirmed; however, the use of citrated blood was demonstrated in the routine work of the clinical microbiology laboratory.

Keywords : sheep citrated blood; sheep dehidratated blood; bacterial growth; hemolysis, bacterial isolation.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )