Revista Cubana de Endocrinología
versión On-line ISSN 1561-2953
GONZALEZ SUAREZ, Roberto M.; ARRANZ CALZADO, María Celeste y GARCIA DAFONTE, Gema. Development of an immunoenzimatic method to determine progesterone. Rev Cubana Endocrinol [online]. 2002, vol.13, n.2, pp. 0-0. ISSN 1561-2953.
An immunoenzimatic, competitive liquid stage method , with separation by antibodies coupled to magnetizable particles was developed to determine progesterone in plasma, It is based on the competence for the binding sites of a monoclonal antibody between the hormone present in the sample and a hormone-enzime conjugate in the presence of blocking agents of the proteins transporting steroids from plasma. The antigen-antibody complex is trapped by a second mouse antiglobulin antibody coupled to magnetizable particles that allow to separate it from the non reacting components of the system and to develop in its surface the color reaction with which the analyte is quantified. This system does not require either extraction of the steroids from plasma with organic solvents or centrifugation and the final product of the reaction may be read in a conventional colorimeter. All this make it accessible to the equipment of a laboratory of conventional clinical biochemistry. The parameters of calibration and quality of both methods were studied and compared with 2 similar commercial analytic systems and with the results of progesterone determination in 200 samples. It was proved that there were no statistical differences between them. It was concluded that this system has all the necessary requirements to be incorporated as a routine method in the laboratories of the National Health System.
Palabras llave : IMMUNOENZIME TECHNIQUES [methods]; PROGESTERONE [analysis]; MONOCLONAL ANTIBODIES [immunology]; OVULATION [immunology].