Resumen

RODRIGUEZ PENDAS, Bertha V. et al. Monoclonal antibodies against human chorionic gonadotropin (HCG) for their use in pregnancy detection. Rev Cubana Endocrinol [online]. 2004, vol.15, n.3. ISSN 1561-2953.

The generation of 2 mouse monoclonal antibodies directed against the human chorionic gonadotropin hormone (CGh), starting from the immunization of BALB/c mice with human CGh purified at the National Institute of Endocrinology (NIEN) is reported. IgG monoclonal antibodies were obtained. They were purified starting from the ascitic fluid by affinity chromatography in protein G Sepharose. The affinity and specificity study showed that these antibodies could be useful in immunoenzimatic assays, using one of them in the microELISA system of our institution for the qualitative detection of pregnancy in urine.

Palabras clave : Hybridoma; glycoprotein hormones; immunoenzymatic assay.