Abstract

JUNCO DIAZ, Raquel de los A.; MARRERO, María Luisa  and  LARA ORTIZ, César. Staphylococcus e infección nosocomial. Rev Cubana Hig Epidemiol [online]. 2000, vol.38, n.1, pp.24-28. ISSN 0253-1751.

We studied a total of 366 strains of Staphylococcus isolated from patients with in-hospital infections from various hospitals throughout the country in 1991-1994 period. Of these, 303 strains were identified as Staphylococci aureus which were characterized using the following epidemiologic markers: phage typing, antibiotic typing and determination of enterotoxin production (A, B, C2, D and E. The most frequent phago types were 47,85 (13,80 %) and 53,54 (10,87 %). 64 strains resulted non-typable in a routine testing dilution (RTD) so, a one-hundred time more concentrated phago dilution, i.,e. 100 x RTD. Was used. Antibiotic typing revealed that the most frequente pattern of resistence was A-P (ampicilline- penicilline) with 37 strains and these two antimicrobial agents were present in 97,7 % of studied strains. The determination of production of enterotoxins A, B, C₂, D and E disclosed that 30 % of strains were enterotoxigenic in which enterotoxin E-producing strains (14 %) predominated. There was an association of enterotoxigenic strains with those stains of phage group III and phage type 94,96. No relationship was found between the patterns of resistance and the identified phage types, which is explained by the small number of strains studied in each hospital. Sixty-three strains were identified as coagulase-negative staphylococci which corresponded to Staphylococci epidemidis. Fifty of them (79,36 %) were slime producers.

Keywords : STAPHYLOCOCCUS [isolation and purification]; STAPHYLOCOCCUS AUREUS [isolation & purification]; CROSS INFECTION.

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