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Revista Cubana de Higiene y Epidemiología

On-line version ISSN 1561-3003


MARTINEZ ROMERO, María Rosarys; GARCIA LEON, Grechen; SARDINA ARAGON, Misleidis  and  MONTORO CARDOSO, Ernesto. Quality control of acid fast bacilli of sputum smears microscopy in tuberculosis provincial reference laboratories in Cuba. Rev Cubana Hig Epidemiol [online]. 2012, vol.50, n.1, pp.29-36. ISSN 1561-3003.

Introduction: The laboratory is a critical component in the diagnosis, treatment, prevention and control of tuberculosis. Objective: To analyze the behavior of quality control of bacilloscopy in provincial reference laboratories of Cuba, by the re-checkup of blind plates. Methods: The quality control was carried out by re-checkup method of plates at 5 424 plates of BAAR sputum received in the National Reference Laboratory of Tuberculosis and Mycobacteria of the "Pedro Kourí" Institute, originating in the laboratories of tuberculosis of Provincial Centers of Hygiene, Epidemiology and Microbiology of Cuba from 2007 to 2009. Results: Fifty four reading errors were identified; from them 20 false-positive, 13 false-negatives and 21 coding error. The sensitivity, specificity and concordance showed values of 84.7, 99.6 and 99.4 %, respectively. The concordance between the NRLTB PKI and the provincial tuberculosis laboratories assessed was of 99.4 % (Kappa rate 0.8105) and the discordance was only of 0.6 %. Conclusions: Results suggest an appropriate staff quality of the provincial laboratories staff to carry out the quality control of BAAR sputum BK. It is recommended to continue with visits to network laboratories to detect the deficiencies and to implement appropriate corrective measures to go on with the diagnosis quality of BK and so to eliminate the TB as a health problem in Cuba.

Keywords : Bacilloscopy; tuberculosis; quality control.

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