Revista Habanera de Ciencias Médicas
versión On-line ISSN 1729-519X
ALVAREZ ALMANZA, Delfín. Identification of extended spectrum â-lactamases in enterobacterias. Rev haban cienc méd [online]. 2010, vol.9, n.4, pp. 516-524. ISSN 1729-519X.
The ß-lactamasses of extended spectrum (ESBL) are enzymes that are phenotypically characterized to confer resistance to penicillin and cephalosporin, including those of third and quarter generation. They are mainly produced by Enterobacteriaceae especially frequent in Klebsiella pneumoniae and Escherichia coli. The identification of this enzyme type is very important because it imposes an action plan for the control of the infections that could take place. Therefore it becomes necessary to differ among the production of betalactamasses of extended spectrum (ESBL) and other resistant mechanisms to avoid the inadequate treatment of infections caused by this type of stumps. The laboratory procedures for the identification of ESBL are very important; there are several phenotype methods, biologic, biochemical and genotype assay. A methodology that joints sensibility, specificity and simplicity, doesn't exist given the complexity of the detection of the ESBL and its variants, thus an appropriate interpretation of the profiles of sensibility is recommended with the habitual approaches of interpreted reading of the antibiogram and later on, the confirmation method should be chosen . What will allow to know the true dimension of the problem that it represents, to limit their dissemination and to adapt the scarce therapeutic options.
Palabras llave : Extended spectrum ß _lactamasses (ESBL); Enterobacteriaceae Identification; Resistance mechanisms; antibiotics.