Resumen

NUNEZ CAIRO, Carlos Rafael. Chronic renal failure model with the use of tisuacryl in rats. Rev haban cienc méd [online]. 2012, vol.11, suppl.5, pp.593-604. ISSN 1729-519X.

Introduction: to provide better health care to renal failure patients is a national health goal in our country; thus, different studies have been carried out using biomodels for renal failure. To improve the reliability of these biomodels still remains as a major challenge. Particularly, models for chronic renal failure (CRF) have been widely used, pursuing a better accuracy in instauration and evolution time, especially in defining the failure's duration. Objective: to validate an experimental model for CRF in rats using Tisuacryl developed in our laboratory. Methods: ninety Wistar rats were distributed into series (of 7, 28, 42, and 60 days) and working groups (controls and experimental) for each series. CRF model for ablation of 5/6 of the renal mass was designed, using the tissue adhesive Tisuacryl (of national production) as sealant of the cut surfaces. The following parameters were evaluated: protein excretion during 24 hours, plasmatic creatinine, renal effective plasmatic flow (REPF), glomerular filtration rate (GFR), and filtration fraction. Results: an increase in protein excretion and creatinine was observed, as well as a drop in, REPF, and GFR. All variables changed to levels compatible with a scenario of CRF of moderate to slight stability level. Conclusions: the Tisuacryl rat model for CRF was evaluated. As result, this model reproduces the conditions of the disease in humans. The best variables for describing the CRF were: protein excretion, plasma creatinine, REPF and GFR, since these allow assessing the failure's degree. In this model the failure degree transits from slight to moderate.

Palabras clave : renal chronic disease; renal chronic failure; model; biomodels; tissue adhesives; cyanocrylates; tisuacryl; rat.

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