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Revista Habanera de Ciencias Médicas
versión On-line ISSN 1729-519X
Resumen
RODRIGUEZ PELIER, Cira Virgen et al. Standardization and validation of an ELISA for quantifying titanic antitoxin in human serum. Rev haban cienc méd [online]. 2013, vol.12, n.4, pp. 0-0. ISSN 1729-519X.
Introduction: the Titanic antitoxin quantification is useful to assess the immune response against vaccinal antigens. Objective: to develop a standardization and validation technique of the indirect ELISA to quantify titanic antitoxin in human serum. Materials and Methods: the standard preparing to the assay curve was calibrated front the preparing Finlay Institute. The optimal indicators were normalized to each technique step developed. Results: a good intra-assay precision was reach during validation in all cases. The coefficient of variation to parallelism was less than 10% and 110%. Detection limit was 0.002 UI/ ml. Analytic sensitivity reached was 0.01 UI/ ml. Conclusions: the assay may be used to quantify Titanic antitoxin in human serum.
Palabras clave : titanic antitoxin; inmuneenzimatic assay; ELISA; validation.