MARTINEZ HERNANDEZ, Carlos M. et al. Study of Methane Potential in Three Bovine Pastures Pre-Treated with Enzymes. []. , 29, 1, e08.   01--2020. ISSN 2071-0054.

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In this work, it was studied the influence of ZY maXX XL 200 enzyme pre-treatment of three agricultural substrata (mulatto pasture, natural pasture and variety CT-115), on their potentiality for methane production. The pre-treatment consisted on the addition of an enzyme (ZY maXX XL 200) in dose of 100 µl/syringes for 350 mg of substrate in 30-1 mL of bovine inoculum. The results of the substrates bio digested were compared with the ones obtained without the addition of the investigated enzyme. In the three cases, significant increments of specific methane yield were achieved. The best result was obtained with mulatto pasture (0.268 m3 CH4/kg VS), followed by the variety CT-115 (0.258 m3 CH4/kg VS) and culminating with natural pasture (0.254 m3 CH4/kg VS). Nevertheless, increments were observed when the pre-treatment was used with enzymes and bovine inoculum vs. when it was not used. On the other hand, the pH had a similar behavior in all substrates where the pre-treatment was applied, as well as where it was not used.

En este trabajo se realizó un estudio del efecto de pre-tratamiento con la enzima (ZY maXX XL 200) a tres diferentes substratos agrícolas (pasto mulato, pasto natural y variedad CT-115) y su influencia sobre el potencial de producción de metano. Se aplicaron dosis de 100 µl/jeringas por 350 mg de substratos en 30-1 mL de inóculo vacuno. Los resultados de los substratos biodigestados fueron comparados con los obtenidos sin la adición de la enzima. En todos los casos, se lograron incrementos significativos en el rendimiento específico de metano. Los mejores resultados se obtuvieron con el pasto mulato (0,268 m3 CH4/kg VS), seguidos del CT-115 (0,258 m3 CH4/kg VS) y culminando con el pasto natural (0,254 m3 CH4/kg VS); no obstante, se observaron incrementos significativos cuando se utilizó el pre-tratamiento con enzimas y un inóculo, comparados con las combinaciones que no fueron aplicados el pre-tratamiento. Por otra parte, se demostró que el pH evolucionó de una manera similar en las biomasas que utilizaron el pre-tratamiento con enzima y sin este.

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