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Biotecnología Vegetal

versión On-line ISSN 2074-8647

Resumen

VAZQUEZ, Dainette; ZAPATA, Mildred  y  BOLANOS, Carlos. Potential of Bacillus pumilus Bp4185 and Bacillus thuringiensis Bt3971 for coffee berry borer biocontrol. Biot. Veg. [online]. 2020, vol.20, n.3, pp.167-176.  Epub 01-Sep-2020. ISSN 2074-8647.

Coffee berry borer (Hypothenemus hampei), is one of the main pests affecting coffee production worldwide. This pest feeds and reproduces inside the berry reducing its weight and quality, causing losses up to 50%. Some strains of Bacillus have plasmids that incorporate cry and cyt genes, whose products have toxic properties to insects. Bacillus thuringiensis and Bacillus pumilus have been evaluated as entomopathogenic. The objectives of this study was to determine the potential of two native Bacillus strains (B. thuringiensis Bt3971 and B. pumilus Bp4185) isolated from coffee ecosystem of Puerto Rico as biocontrol of coffee berry borer. The presence of a 12 kbp plasmid was confirmed in Bt3971 and Bp4185, and a 116 kDa protein with entomopathogenic potential from Bt3971. After 48 h of treatment, Bt3971 (A590=0.8, approximately 1010 CFU ml-1) caused 55% mortality and 82% at 72 h in a population of insects. Bp4185 strain, at 72 h caused 55% mortality (A590=1.0, approximately 1012 CFU ml-1) and 46% at 96 h (A590=0.8). Both native bacterial strains, adapted to the environmental conditions of Puerto Rico, have potential for biological control of coffee berry borer.

Palabras clave : cry1A; Hypothenemus hampei; plasmids; toxicity.

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