Resumo

BRUNETTO DE GALLIGNANI, María del Rosario et al. Development of an analytical Method for the Determination of Glucose, Fructose and Sucrose in Venezuelan Cocoa Samples. Rev Cub Quim [online]. 2014, vol.26, n.3, pp.181-201. ISSN 2224-5421.

A selective and sensitive gas chromatography method with mass spectrometric detection (GC/MS) was optimized and validated for the determination of fructose , glucose and sucrose in cocoa beans. Analytes were extracted by ultrasonication from defatted milled cocoa powder using purified water (18 Ω) at 70 ^oC. Then, these were derivatized with N-methyl-trimethylsilyl trifluoacetamida (MSTFA) assisted by microwave irradiation at a power of 630 W for 60 s, in order to achieve its volatility. Under optimal conditions , the method provided quantitative recoveries of analytes (98-108 %, CV < 3,68 % n = 5) and a precision with a CV < 3,31 % (n = 5). Ninety samples of four varieties, with different genotypes, of cocoa beans grown in Venezuela , taken before, during and after the fermentation and roasting stages, were analyzed.

Palavras-chave : MS; microwaves; cocoa; glucose; fructose; sucrose.

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