Resumo

HEREDIA-DIAZ, Yamilé et al. Serum and plasma samples deproteinization for analytical studies of carbamazepine. Rev Cub Quim [online]. 2016, vol.28, n.3, pp.870-889. ISSN 2224-5421.

The quantitative determination of carbamazepine in human serum and plasma, it requires of a previous treatment to eliminate the proteins. In this study were used methanol and trichloroacetic acid to 10 %, with the purpose of selecting the protein precipitant that guarantees the biggest effectiveness to remove the proteins. The proportions serum/plasma-protein precipitant 1:2 for the methanol and 1:0,2 for the trichloroacetic acid were used. The evaluation of the efficiency of protein precipitation was carried out applying UV spectrophotometry; the spectra of the precipitated samples were registering in the range of 200-340 nm. The selection approach was the decrease from the absorbance intensity to the wave lengths of maximum absorption of the proteins. The methanol guaranteed a reduction of the absorbance in the region near at 235 nm and 280 nm as much in the serum as in the plasma. The trichloroacetic acid 10% didn't show effectiveness to remove the proteins.

Palavras-chave : Carbamazepine; quantitative determination; serum; plasma; UV- spectrophotometry.

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