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Revista Cubana de Química

On-line version ISSN 2224-5421

Abstract

NARCIANDI-DIAZ, Ramón Emilio  and  RIVERA-MARTIN, Juan Miguel. Production of Gp-41 (HIV-1) recombinant protein in E.coli: effect of magnetic field. Rev Cub Quim [online]. 2020, vol.32, n.1, pp.3-19.  Epub Feb 26, 2020. ISSN 2224-5421.

The influence of the extremely low frequency electromagnetic field was studied in a fed batch culture of Escherichia coli (E.coli), developed for the production of the recombinant Gp-41 protein of human immunodeficiency virus type 1 (HIV-1). An electromagnet, which generates non-homogeneous magnetic fields, was used, at a constant induction value of 0.055 Tesla, and exposure time of 6, 5 h. With the application of the magnetic field, a 1,2-fold increase in volumetric production (320 mg / L) and 1,5 times in process productivity (30 mg / L) were obtained. At the same time, acetate production decreased 20 times to values ​​of 0, 47 g / L, while the expression level of Gp-41 protein increased to 10 %.

Keywords : E. coli; Gp-41; HIV-1; fed batch culture; magnetic field.

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