Resumen

BALBIN-TAMAYO, Abel I. et al. The usefulness of biotinylated h1gag1584 gag gene probe to label-free electrochemical detection proviral DNA for HIV-1. Rev Cub Quim [online]. 2021, vol.33, n.3, pp.367-382.  Epub 26-Oct-2021. ISSN 2224-5421.

The aim of the study is to demonstrate that the biotinylated 19 base oligonucleotide probe of the gag gene H1Gag1584 can be used as a molecular recognition element for the detection of HIV-1 proviral DNA, in two electrochemical detection systems of free laber. Two free leber electrochemical detection systems were evaluated: an impedimetric DNA genosensor using a self-assembled biotin monolayer (SAM) and a voltammetric DNA genosensor by modifying a new epoxy graphite transducer with graphene oxide. The results obtained in this study showed that the biotinylated 19-base oligonucleotide probe of the gag gene H1Gag1584 can be used as a molecular recognition element for the detection of HIV-1 proviral DNA, in two electrochemical detection systems of free labels.

Palabras clave : probe H1Gag1584; Graphite-epoxy; Gold; Genosensor; HIV-1; DNA.

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