Abstract

RAMOS-SANCHEZ, Luis B.; ALCALA GALIANO-MORELL, Diana D.; SANCHEZ-VARGAS, Héctor E.  and  RIZO-PORRO, Mariela. In silico analysis of a mammalian cell culture in two stage fed-batch bioreactors. RTQ [online]. 2017, vol.37, n.3, pp.529-545. ISSN 2224-6185.

The objective of this work is to compare a two-stage fed-batch culture system of mammalian cells with a single culture system. In silico process-design techniques were used in order to intensify the production of monoclonal antibodies in both cases before comparing them. As case study a structured kinetic model reported in literature for an industrial process of monoclonal antibodies (MAb) harvested from cultures of hybridoma cells was selected. Computational simulation was carried-out using MATLAB software as computing platform. Results demonstrated that in this particular case a two-stage fed-batch culture system produces higher productivities in MAb production compared to simple stage systems. In double stage systems, having a fraction of volume of the first stage of 80 %, an increment of 58 % in MAb concentrations and 30 % in productivity were obtained when compared to a single stage system.

Keywords : Cell culture; monoclonal antibodies; optimization; two-stage semi-continuous bioreactor.

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