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Vaccimonitor

Print version ISSN 1025-028XOn-line version ISSN 1025-0298

Vaccimonitor vol.9 no.3 Ciudad de la Habana July-Sept. 2000

 

ARTICULOS ORIGINALES

 

Guía para la estandarización de técnicaGuía técnicas inmunoenzimáticas en ensayos de vacunas

 

Guide for the standardization of enzyme immunoassays for use in vaccine trials.

 

Rolando Ochoa, Juan C. Martínez, Xenia Ferriol, Eric Estrada, Ana M. García, Rosa Blanco, Franklin Sotolongo.

Instituto Finlay. Centro de Investigación-Producción de Vacunas y Sueros. Ciudad de La Habana, Cuba.
E-mail:ochoa@finlay.edu.cu


RESUMEN

Se realizó una revisión sobre la estandarización de técnicas inmunoenzimáticas para ensayos preclínicos y
clínicos de vacunas, elaborándose una guía práctica. Se analizan los principios a usar y los pasos a seguir para
la optimización de un ensayo, incluyendo la preparación de estándares y controles. Se exponen nuestras
experiencias.

Palabras claves: Estandarización, inmunoensayos enzimáticos, ELISA, vacunas.


ABSTRACT

The standardization of enzyme immunoassays, for use in evaluation of pre-clinical and clinical vaccine trials, was reviewed. A practical guide was made. The immunoassay principles and the assay optimization step – including the preparation of standards and controls – are analyzed. Our experience is presented.

Key words: Standardization, enzyme immunoassays, ELISA, vaccines.


texto completo en pdf

REFERENCIAS

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2. Martínez JC, Ochoa R, Cruces A, Fajardo EM, Alvarez E, Ferriol X et al. Validation of an ELISA for the Quantitation of Diphtheria Antitoxin in Human Serum. Biotecnología Aplicada. 2000;17:183-186.

3. Ann NQ, Hong HA, Nhon TN, Think ND, Van NT, Hendriks J. Tetanus antibodies measured by the toxin binding inhibition test (ToBI) in mothers and children in the Neonatal Tetanus Program in Vietnam. Dev Biol Stand 1999;101:247-253.

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