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Vaccimonitor

Print version ISSN 1025-028XOn-line version ISSN 1025-0298

Vaccimonitor vol.13 no.3 Ciudad de la Habana Sept.-Dec. 2004

 

ARTICULOS ORIGINALES

 

Caracterización fenotípica y serológica de aislamientos clínicos de Pseudomonas aeruginosa.

 

Phenotypical and Serological Characterizations of Pseudomonas aeruginosa Clinical Isolates.


Adriana Callicó, Bárbara Cedré, Sergio Sifontes, Vismar Torres, Yadira Pino, Ana H. Callís, Sara C. Esnard.

 

Instituto Finlay. Centro de Investigación-Producción de Vacunas y Sueros. Ciudad de La Habana. Cuba. E-mail: bcedre@finlay.edu.cu


RESUMEN

Generalmente, Pseudomona. aeruginosa es considerada un patógeno oportunista. Bajo ciertas condiciones esta bacteria puede infectar diferentes tipos de heridas, particularmente quemaduras, pero también el tracto urinario y respiratorio. Esta bacteria se asocia con neumonía, endocarditis, meningitis y algunas cepas producen grandes cantidades de polisacárido extracelular, las que se asocian a casos de fibrosis quística. El objetivo de este estudio fue caracterizar cepas clínicas aisladas de pacientes con diferentes patologías con el fin de seleccionar cepas a partir de las cuales obtener antígenos candidatos a una vacuna por subunidades. Los serotipos de mayor frecuencia encontrados fueron el O1, O4, O6, O9 y O11 y se halló una relación entre el serotipo O9 y los casos de fibrosis quística. También se evaluó la virulencia de las mismas mediante el ensayo de siembra en rojo congo. El 72% de las 18 cepas evaluadas produjeron colonias blancas en más del 50% del total de colonias, lo que indica que la mayoría de nuestras cepas son virulentas, ya que este ensayo es una medida indirecta de la capacidad de producir alginato, el cual es considerado un importante factor de virulencia.

Palabras claves: Caracterización fenotípica, serotipaje, Pseudomonas aeruginosa, virulencia.


ABSTRACT

The aim of this study was to characterize P. aeruginosa clinical strains isolated from patients with different pathologies in order to select the strains for obtaining candidate antigens for subunit vaccines. The most common serotypes found were O1, O4, O6, O9 and O11. We found a relation between O9 serotype and cystic fibrosis patients. The ability to produce white colonies was also studied by Congo red assay. Seventy two percent of 18 evaluated strains produced white colonies in more than 50% of the total of colonies, which indicates the most of our strains are virulent because this assay is an indirect measurement of the capacity to produce alginate, considered an important virulence factor.

Keywords: Phenotypical characterization, serotyping, Pseudomonas aeruginosa, virulence.


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