<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0864-0394</journal-id>
<journal-title><![CDATA[Pastos y Forrajes]]></journal-title>
<abbrev-journal-title><![CDATA[Pastos y Forrajes]]></abbrev-journal-title>
<issn>0864-0394</issn>
<publisher>
<publisher-name><![CDATA[Estación Experimental de Pastos y Forrajes Indio Hatuey]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0864-03942019000300218</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Cuantificación de prolina libre en la dieta del ganado meliponícola de las provincias de Matanzas y Mayabeque]]></article-title>
<article-title xml:lang="en"><![CDATA[Quantification of free proline in the diet of the stingless bee livestock of the Matanzas and Mayabeque provinces]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Fonte-Carballo]]></surname>
<given-names><![CDATA[Leydi]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[González-Paramás]]></surname>
<given-names><![CDATA[Ana María]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Rodríguez-de la Cruz]]></surname>
<given-names><![CDATA[David]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Díaz-Solares]]></surname>
<given-names><![CDATA[Maykelis]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Morales-Querol]]></surname>
<given-names><![CDATA[Dariel]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[González-Sierra]]></surname>
<given-names><![CDATA[Liliet]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Altunaga-Pérez]]></surname>
<given-names><![CDATA[Nancy]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Castro-Cabrera]]></surname>
<given-names><![CDATA[Inelvis]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Lugo-Morales]]></surname>
<given-names><![CDATA[Yudit]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Ministerio de Educación Superior Universidad de Matanzas Estación Experimental de Pastos y Forrajes Indio Hatuey]]></institution>
<addr-line><![CDATA[Matanzas ]]></addr-line>
<country>Cuba</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Universidad de Salamanca Departamento de Nutrición y Bromatología Facultad de Farmacia]]></institution>
<addr-line><![CDATA[España ]]></addr-line>
</aff>
<aff id="Af3">
<institution><![CDATA[,Universidad de Salamanca Departamento de Botánica y Fisiología Vegetal ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>España</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>09</month>
<year>2019</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>09</month>
<year>2019</year>
</pub-date>
<volume>42</volume>
<numero>3</numero>
<fpage>218</fpage>
<lpage>222</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_arttext&amp;pid=S0864-03942019000300218&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_abstract&amp;pid=S0864-03942019000300218&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_pdf&amp;pid=S0864-03942019000300218&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen Una nutrición óptima mediante el consumo de una dieta equilibrada, rica en aminoácidos, es imprescindible para el buen funcionamiento del sistema inmune de los animales. Por ello, este trabajo tuvo como objetivo determinar la cantidad de prolina libre, presente en los alimentos consumidos (polen y miel) por el ganado meliponícola en las provincias de Matanzas y Mayabeque. Para realizar la determinación de prolina libre mediante espectrofotometría, se tomaron 60 g de polen, provenientes de torales sellados, y 80 mL de miel en cada una de las diez colmenas muestreadas (cinco por provincia), elegidas al azar. Después de verificar que los datos cumplieron con los supuestos de homogeneidad de varianza y distribución normal, se procedió a realizar un análisis de varianza simple. Las medias se compararon por el test de Duncan, para un nivel de significación de 5 %. Para todos los análisis se utilizó el paquete estadístico Infostat®, versión 1.1. Los resultados indicaron que, desde el punto de vista estadístico, la cantidad de prolina libre encontrada en las mieles procedentes de ambas provincias, difirió significativamente. Mientras, los valores de prolina libre hallados en el polen procedente de Matanzas, estuvieron en el rango de 4,2 a 19,5 mg/g. El que procedió de Mayabeque presentó valores de 5,9 a 7,1 mg/g. Se concluye que las mieles, como los pólenes, que constituyen la fuente de alimentación del ganado meliponícola de ambas provincias, cuentan con una proporción adecuada de prolina libre. Por tanto, la masa ganadera ubicada en ambas zonas tiene el soporte aminoacídico necesario en su dieta para desarrollar una respuesta inmune adecuada ante cualquier agente microbiano.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract An optimum nutrition through the consumption of a balanced amino acid-rich diet is essential for the good functioning of the immune system of animals. For such reason, the objective of this work was to determine the quantity of free proline, present in the consumed feedstuffs (pollen and honey) by the stingless bee livestock in the Matanzas and Mayabeque provinces. To determine the free proline through spectrophotometry, 60 g of pollen were taken, from sealed amphorae, and 80 mL of honey in each of the ten randomly chosen sampled beehives (five per province). After verifying that the data fulfilled the assumptions of variance homogeneity and normal distribution, a simple variance analysis was performed. The means were compared by Duncan&#8217;s test, for a significance level of 5 %. For all the analyses the statistical package Infostat®, version 1.1, was used. The results indicated that, from the statistical point of view, the quantity of free proline found in the honeys from both provinces significantly differed. Meanwhile, the free proline values found in the pollen from Matanzas, were in the range from 4,2 to 19,5 mg/g. The one from Mayabeque showed values from 5,9 to 7,1 mg/g. It is concluded that the honeys, like the pollens, which constitute the feeding source of the stingless bee livestock of both provinces, have an adequate proportion of free proline. Thus, the livestock located in both zones has the necessary amino acid support in its diet to develop adequate immune response to any microbial agent.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[aminoácidos]]></kwd>
<kwd lng="es"><![CDATA[miel]]></kwd>
<kwd lng="es"><![CDATA[polen.]]></kwd>
<kwd lng="en"><![CDATA[amino acids]]></kwd>
<kwd lng="en"><![CDATA[honey]]></kwd>
<kwd lng="en"><![CDATA[pollen]]></kwd>
</kwd-group>
</article-meta>
</front><back>
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