TIPIFICATION BY CAPSULAR REACTION TEST of Streptococcus suis ISOLATED IN PIGS GENOTYPES cps 2J+ AND cps 9J+

TIPIFICACIÓN MEDIANTE PRUEBA DE LA REACCIÓN CAPSULAR DE Streptococcus suis AISLADO EN GENOTIPOS DE CERDOS cps 2J+ Y cps 9J+

Dear Editor:

Streptococcus suis is a high pathological gram-positive bacterium infecting pigs. In this species, 33 capsular serotypes are recognized; however the most prevalent are serotypes 1/2, 2, 3, 7, 9 and 14, but the distribution of predominant serotypes may vary in different geographic regions. Serotype 2 is the most pathogenic and shows an elevated zoonotic potential. ]]> Our lab has worked on the obtaining of specific antiserum to capsular genotype cps 2J+ and cps 9J+ of Streptococcus suis from clinical isolates associated to pathological lesions of swine respiratory tract.

A rapid diagnostic based agglutination test which allows an easy, rapid, cost-effective and specific discrimination among different streptococcus and others related with porcine respiratory complex bacterial strains have been assayed.

Both antisera (vs-cps 2 and vs-cps 9) recognized the homologue strain with titer of 1/32 and did not show a positive reaction with other bacteria associated to pig respiratory complex, sush as Mycoplasm hyopneumoneae, Pasteurella multocida, other genotypes of Streptococcus suis used in this study, as well as Streptococcus equisimilis (zooepideremicus) and Mycoplasm hyorini.

For further studies, it necessary to increase the number of isolates. More specific and sensitive techniques for diagnosis of genotype cps 2J+ and cps 9 will be assayed directly in clinical samples as organs with invasive procedures, nasal and bronchial swabs.

M. Baez, Ivette Espinosa, Siomara Martínez, J. Vichi

Laboratory of Bacteriology, Molecular Biology Group, Department of Microbiology, CENSA, Cuba. E-mail: michel@censa.edu.cu ]]>