<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0253-570X</journal-id>
<journal-title><![CDATA[Revista de Salud Animal]]></journal-title>
<abbrev-journal-title><![CDATA[Rev Salud Anim.]]></abbrev-journal-title>
<issn>0253-570X</issn>
<publisher>
<publisher-name><![CDATA[Centro Nacional de Sanidad Agropecuaria]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0253-570X2020000300009</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Detección de Streptococcus agalactiae a partir de muestras de leche cruda]]></article-title>
<article-title xml:lang="en"><![CDATA[Detection of Streptococcus agalactiae from raw milk samples]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Ledesma Rodríguez]]></surname>
<given-names><![CDATA[Anel]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Díaz Herrera]]></surname>
<given-names><![CDATA[Dervel Felipe]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Ribot Enrique]]></surname>
<given-names><![CDATA[Ariel]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Ramón Díaz]]></surname>
<given-names><![CDATA[Dianis]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Martínez Vasallo]]></surname>
<given-names><![CDATA[Ailín]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Uffo Reinosa]]></surname>
<given-names><![CDATA[Odalys]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Centro Nacional de Sanidad Agropecuaria (CENSA) Laboratorio de Ensayos para el Control de la Calidad de los Alimentos (CENLAC) ]]></institution>
<addr-line><![CDATA[San José de las Lajas Mayabeque]]></addr-line>
<country>Cuba</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>12</month>
<year>2020</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>12</month>
<year>2020</year>
</pub-date>
<volume>42</volume>
<numero>3</numero>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_arttext&amp;pid=S0253-570X2020000300009&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_abstract&amp;pid=S0253-570X2020000300009&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_pdf&amp;pid=S0253-570X2020000300009&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[RESUMEN Streptococcus agalactiae (S. agalactiae) es uno de los microorganismos más importantes asociados a la mastitis bovina, enfermedad que provoca grandes pérdidas económicas y constituye un riesgo para la salud animal y humana. El cultivo microbiológico es la &#8220;técnica de oro&#8221; para el diagnóstico de S. agalactiae, sin embargo, requieren un trabajo intenso para obtener los resultados. Una alternativa a las pruebas microbiológicas es la detección de S. agalactiae mediante la amplificación por la Reacción en Cadena de la Polimerasa (PCR) a partir de ADN extraído de leche cruda, lo que disminuye el tiempo de diagnóstico del patógeno y reduce la aparición de resultados falsos positivos asociados al cultivo. El objetivo de este trabajo fue la detección de S. agalactiae por PCR a partir de ADN extraído de muestras de leche cruda. La extracción de ADN se realizó por extracción orgánica; paralelamente, se realizó el cultivo microbiológico de las muestras y su posterior análisis por la prueba de Christie-Atkins-Munch-Peterson (CAMP). Para el ensayo de PCR se utilizaron cebadores que amplifican un fragmento de 153pb del gen cfb, que codifica para el factor CAMP. Un total de 13 muestras resultaron positivas a la prueba de CAMP. El ensayo de PCR amplificó el ADN extraído en 10 de las 50 muestras, que coincidieron con las muestras positivas del ensayo de CAMP. Tres muestras positivas a CAMP resultaron negativas por PCR. El uso del PCR permitió la detección de S. agalactiae en vacas con mastitis.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[ABSTRACT Streptococcus agalactiae (S. agalactiae) is one of the most important microorganisms associated with bovine mastitis, a disease that causes great economic losses and represents a risk for human and animal health. Microbiological cultures constitute the "gold standard technique" for the diagnosis of S. agalactiae; however, they require intensive work to obtain the results. An alternative to the microbiological testing is the detection of S. agalactiae through amplification by Polymerase Chain Reaction (PCR) from DNA extracted from raw milk, which reduces the diagnosis time of the pathogen and decreases the occurrence of false positive results associated with the culture. The objective of this work was the identification of S. agalactiae by PCR from DNA extracted from raw milk samples. DNA extraction was performed by organic extraction. In parallel, the microbiological culture of the samples was carried out and their subsequent analysis by the Christie-Atkins-Munch-Peterson test (CAMP). For the PCR assay, primers amplifying a 153pb fragment of the cfb gene, encoding for the CAMP factor, were used. Thirteen samples were positive to the CAMP test. The PCR assay amplified the extracted DNA in 10 of the 50 samples, which coincided with the positive samples of the CAMP test. Three samples positive to CAMP were negative by PCR. The use of PCR allowed the detection of S. agalactiae in cows with mastitis.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Streptococcus agalactiae]]></kwd>
<kwd lng="es"><![CDATA[leche]]></kwd>
<kwd lng="es"><![CDATA[mastitis]]></kwd>
<kwd lng="es"><![CDATA[PCR]]></kwd>
<kwd lng="en"><![CDATA[Streptococcus agalactiae]]></kwd>
<kwd lng="en"><![CDATA[milk]]></kwd>
<kwd lng="en"><![CDATA[mastitis]]></kwd>
<kwd lng="en"><![CDATA[PCR]]></kwd>
</kwd-group>
</article-meta>
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