<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0253-5785</journal-id>
<journal-title><![CDATA[Centro Agrícola]]></journal-title>
<abbrev-journal-title><![CDATA[Ctro. Agr.]]></abbrev-journal-title>
<issn>0253-5785</issn>
<publisher>
<publisher-name><![CDATA[Editorial Feijóo, Universidad Central de Las Villas]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0253-57852020000500063</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Identificación de bacterias endófitas formadoras de endosporas asociadas a Theobroma cacao L. en Quinindé, Esmeraldas, Ecuador]]></article-title>
<article-title xml:lang="en"><![CDATA[Identification of endophytes endospore-forming bacteria associated to Theobroma cacao L. in Quininde, Esmeraldas, Ecuador]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Vera-Loor]]></surname>
<given-names><![CDATA[María Aracely]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Bernal Cabrera]]></surname>
<given-names><![CDATA[Alexander]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Vera-Coello]]></surname>
<given-names><![CDATA[Danilo]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Leiva-Mora]]></surname>
<given-names><![CDATA[Michel]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Díaz de Villegas]]></surname>
<given-names><![CDATA[Lisbeth Morales]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Instituto Nacional de Investigaciones Agropecuarias Estación Experimental Tropical Pichilingue ]]></institution>
<addr-line><![CDATA[Cantón Mocache Los Ríos]]></addr-line>
<country>Ecuador</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Universidad Central &#8220;Marta Abreu&#8221; de Las Villas Facultad de Ciencias Agropecuarias Departamento de Agronomía]]></institution>
<addr-line><![CDATA[Santa Clara Villa Clara]]></addr-line>
<country>Cuba</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,Universidad Técnica de Ambato Facultad de Ciencias Agropecuarias Laboratorio de Biotecnología]]></institution>
<addr-line><![CDATA[Cevallos Tungurahua]]></addr-line>
<country>Ecuador</country>
</aff>
<aff id="Af4">
<institution><![CDATA[,Universidad Central &#8220;Marta Abreu&#8221; de Las Villas Facultad de Ciencias Agropecuarias Departamento de Biología]]></institution>
<addr-line><![CDATA[Santa Clara Villa Clara]]></addr-line>
<country>Cuba</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>00</month>
<year>2020</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>00</month>
<year>2020</year>
</pub-date>
<volume>47</volume>
<fpage>63</fpage>
<lpage>67</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_arttext&amp;pid=S0253-57852020000500063&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_abstract&amp;pid=S0253-57852020000500063&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_pdf&amp;pid=S0253-57852020000500063&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[RESUMEN El presente trabajo tuvo como objetivo, identificar morfológica, fisiológica y molecularmente bacterias endófitas formadoras de endosporas asociadas a Theobroma cacao (cultivar Criollo tipo Nacional) en el cantón Quinindé, provincia Esmeraldas, Ecuador. La caracterización de las bacterias desde el punto de vista cultural, morfológico y fisiológico se realizó de acuerdo a metodologías recogidas en la literatura científica. La confirmación de la identidad de las bacterias se realizó a partir de la secuenciación del gen que codifica para el ARNr 16S. Todas las cepas mostraron características culturales, morfológicas y fisiológicas semejantes al género Bacillus. La secuenciación confirmó los criterios anteriores y las principales especies identificadas fueron Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus mycoides y Bacillus cereus con un 99 % de similitud; mientras otras cepas no produjeron una secuencia consenso óptima, quedando como Bacillus sp.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[ABSTRACT The aim of this work was to characterize morphologically, physiologically and molecularly endophytic endospore-forming bacteria associated to Theobroma cacao (cv. Criollo type National) in Quininde canton, Esmeraldas province, Ecuador. The bacteria were characterized from the cultural, morphological and physiological point of view according to methodologies collected in the scientific literature. The confirmation of the identity of the bacteria was made from the sequencing of the gene encoding 16S rRNA. As results, all the strains showed cultural, morphological and physiological characteristics similar to the Bacillus genus. The sequencing confirmed the previous criteria and the main species identified were Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus mycoides and Bacillus cereus with 99 % similarity; while other strains did not produce an optimal consensus sequence, remaining as Bacillus sp.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Bacillus spp.]]></kwd>
<kwd lng="es"><![CDATA[cacao]]></kwd>
<kwd lng="es"><![CDATA[caracterización]]></kwd>
<kwd lng="en"><![CDATA[Bacillus spp.]]></kwd>
<kwd lng="en"><![CDATA[cocoa]]></kwd>
<kwd lng="en"><![CDATA[characterization]]></kwd>
</kwd-group>
</article-meta>
</front><back>
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