<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0258-5936</journal-id>
<journal-title><![CDATA[Cultivos Tropicales]]></journal-title>
<abbrev-journal-title><![CDATA[cultrop]]></abbrev-journal-title>
<issn>0258-5936</issn>
<publisher>
<publisher-name><![CDATA[Ediciones INCA]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0258-59362020000100004</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Conservación in vitro de cafeto (Coffea arabica L.) mediante la disminución de sales minerales en el medio de cultivo]]></article-title>
<article-title xml:lang="en"><![CDATA[In vitro conservation of coffee-tree (Coffea arabica L.) by decreasing mineral salts in the culture medium]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Castilla-Valdés]]></surname>
<given-names><![CDATA[Yanelis]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[González-Vega]]></surname>
<given-names><![CDATA[María Esther]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Espinosa-Torres]]></surname>
<given-names><![CDATA[Llayma]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Instituto Nacional de Ciencias Agrícolas (INCA)  ]]></institution>
<addr-line><![CDATA[San José de las Lajas Mayabeque]]></addr-line>
<country>Cuba</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>03</month>
<year>2020</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>03</month>
<year>2020</year>
</pub-date>
<volume>41</volume>
<numero>1</numero>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_arttext&amp;pid=S0258-59362020000100004&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_abstract&amp;pid=S0258-59362020000100004&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_pdf&amp;pid=S0258-59362020000100004&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[RESUMEN La conservación de recursos fitogenéticos reviste gran importancia en tiempos en que se agudizan las consecuencias del cambio climático global. El género cafeto (Coffea spp) no está exento de estos riesgos, por lo que se buscan alternativas para su conservación ex situ. Específicamente, los métodos de conservación in vitro conocidos como de &#8216;crecimiento mínimo&#8217;, permiten la preservación a mediano plazo del material vegetal, mediante la disminución del ritmo de crecimiento y de la frecuencia entre subcultivos. Son necesarios estudios acerca del efecto independiente de variaciones en la concentración de macro y microelementos esenciales, en la conservación a mediano plazo de cafeto. El objetivo del trabajo fue determinar el efecto de la disminución del contenido mineral del medio de cultivo en la respuesta de plantas de cafeto (Coffea arabica L.) conservadas in vitro por un período entre dos y seis meses. Las plantas, previamente obtenidas in vitro, fueron sembradas en medio MS modificado, con tres tratamientos consistentes en la reducción al 75, 50 y 25 % de sus macro y microelementos. La supervivencia, el número de pares de hojas, la abscisión foliar y el porcentaje de plantas con raíces fueron evaluados a los dos, cuatro y seis meses. En el tratamiento de MS al 50 %, los porcentajes de supervivencia variaron entre el 85 y 100 %, se obtuvieron valores intermedios de pares de hojas y de abscisión foliar, con respecto al resto de los tratamientos. Además, las plantas desarrollaron raíces que les permitieron sobrevivir a pesar de la carencia de nutrientes. Con este tratamiento, que representa un ahorro de la mitad de los componentes minerales del medio de cultivo, se considera factible conservar por un período de hasta seis meses las plantas in vitro de cafeto.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[ABSTRACT The importance of the conservation of plant genetic resources is becoming higher at times when the consequences of global climate change are more acute. The coffee genus (Coffea spp) is not exempt from these risks, so it is researched some alternatives for its ex situ conservation. Specifically, the in vitro conservation methods known as 'slow growth' allow the medium-term preservation of plant material, by decreasing the growth rate and the frequency between subcultures. Studies are needed on the independent effect of variations in the concentration of essential macro and microelements in the medium-term preservation of coffee. The objective of the work was to determine the effect of the decrease of the mineral content of the culture medium in the response of in vitro coffee plants (Coffea arabica L.) preserved by a period between two and six months. The plants, previously obtained in vitro, were cultured in modified MS medium, with three treatments consisting in the reduction to 75, 50 and 25 % of their macro and microelements. The survival, the number of leaf pairs, the leaf abscission and the percentage of rooted plants were evaluated at two, four and six months. In the treatment of 50 % MS, the survival percentages varied between 85 and 100 %, and were obtained intermediate values of leaf pairs and leaf abscission, with respect to the rest of the treatments. In addition, the plants developed roots that allowed them to survive despite the lack of nutrients. With this treatment, which represents a saving of the half of the mineral components of the culture medium, it is considered feasible to preserve the coffee plants for a period up to six months.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[brotes]]></kwd>
<kwd lng="es"><![CDATA[cultivo in vitro]]></kwd>
<kwd lng="es"><![CDATA[nutrientes minerales]]></kwd>
<kwd lng="es"><![CDATA[plántulas in vitro]]></kwd>
<kwd lng="es"><![CDATA[recursos genéticos vegetales]]></kwd>
<kwd lng="en"><![CDATA[in vitro culture]]></kwd>
<kwd lng="en"><![CDATA[in vitro plantlets]]></kwd>
<kwd lng="en"><![CDATA[mineral nutrients]]></kwd>
<kwd lng="en"><![CDATA[plant genetic resources]]></kwd>
<kwd lng="en"><![CDATA[shoots]]></kwd>
</kwd-group>
</article-meta>
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