<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0375-0760</journal-id>
<journal-title><![CDATA[Revista Cubana de Medicina Tropical]]></journal-title>
<abbrev-journal-title><![CDATA[Rev Cubana Med Trop]]></abbrev-journal-title>
<issn>0375-0760</issn>
<publisher>
<publisher-name><![CDATA[Centro Nacional de Información de Ciencias Médicas]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0375-07602021000300004</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Evaluación preliminar de la actividad antiviral de Ageratina havanensis frente al virus dengue-2 utilizando ensayos inmunoenzimáticos]]></article-title>
<article-title xml:lang="en"><![CDATA[Preliminary evaluation of the antiviral activity of Ageratina havanensis against dengue-2 virus using enzyme immunoassays]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Pupo Antúnez]]></surname>
<given-names><![CDATA[Maritza]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Medero Díaz]]></surname>
<given-names><![CDATA[Dailyn]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Vázquez Ramudo]]></surname>
<given-names><![CDATA[Susana]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Morier Díaz]]></surname>
<given-names><![CDATA[Luis F.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Álvarez Vera]]></surname>
<given-names><![CDATA[Mayling]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[del Barrio Alonso]]></surname>
<given-names><![CDATA[Gloria]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad de La Habana Facultad de Biología ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Cuba</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Instituto de Medicina Tropical &#8220;Pedro Kourí&#8221; (IPK)  ]]></institution>
<addr-line><![CDATA[ La Habana]]></addr-line>
<country>Cuba</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>12</month>
<year>2021</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>12</month>
<year>2021</year>
</pub-date>
<volume>73</volume>
<numero>3</numero>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_arttext&amp;pid=S0375-07602021000300004&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_abstract&amp;pid=S0375-07602021000300004&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_pdf&amp;pid=S0375-07602021000300004&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[RESUMEN  Introducción:  Se presenta la evaluación preliminar de la actividad antiviral de extractos de Ageratina havanensis (etanólico de tallo, AH-T-EtOH; butanólico de hoja, AH-H-ButOH y acetato de etilo de hoja, AH-H-AcEtO) utilizando dos sistemas inmunoenzimáticos, un ELISA celular (ELISAc) y la técnica de inmunoperoxidasa. Objetivo: Evaluar la actividad antiviral de los extractos de Ageratina havanensis utilizando dos sistemas inmunoenzimáticos.  Métodos:  Se normalizó y empleó un ELISAc y la técnica de inmunoperoxidasa en evaluar de forma preliminar la actividad antiviral de tres extractos de Ageratina havanensis a diferentes concentraciones y tiempos de adición mediante la detección de la expresión de la proteína E del virus dengue.  Resultados:  El ELISAc logró como parámetros óptimos: línea celular Vero, antígeno viral en cultivo de células, tiempo de expresión de la proteína E de 96 h, compuesto fijador metanol-acetona y bloqueador leche descremada al 1 %. La menor expresión de la proteína E (mayor inhibición sobre la replicación viral), fue al adicionar el extracto AH-T-EtOH a su mayor concentración y 1 h antes de inocular el virus dengue-2 cepa A15 (VDEN-2 A15). El extracto AH-H-ButOH a concentraciones de 125 µg/mL y 250 µg/mL presentó una ligera limitación de expresión de E 1 h después de la inoculación viral. El extracto AH-H-AcEtO a las concentraciones empleadas, no mostró difererencia añadido antes y después de la inoculación. El ensayo de inmunoperoxidasa exhibió resultados análogos para los extractos.  Conclusiones:  La mayor inhibición de la replicación viral fue obtenida con el extracto AH-H-ButOH, a su mayor concentración y ambos tiempos de adición. Los ensayos inmunoenzimáticos aplicados son herramientas útiles para evaluar extractos de Ageratina havanensis con posible actividad antiviral.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[ABSTRACT  Introduction:  A preliminary evaluation is presented of the antiviral activity of Ageratina havanensis extracts (stem ethanolic, AH-T-EtOH; leaf butanolic, AH-H-ButOH; and leaf ethyl acetate, AH-H-AcEtO) using two enzyme immunoassays, cellular ELISA (C-ELISA) and immunoperoxidase technique.  Objective:  Evaluate the antiviral activity of Ageratine havanensis extracts using two enzyme immunoassays.  Methods:  C-ELISA and immunoperoxidase technique were standardized for use in the preliminary evaluation of the antiviral activity of three Ageratina havanensis extracts at different concentrations and addition times through detection of the expression of the E protein of dengue virus.  Results:  C-ELISA achieved the following optimal parameters: Vero cell line, viral antigen in cell culture, protein E expression time 96 h, methanol-acetone fixation compound and 1% skimmed milk blocker. The smallest expression of protein E (greatest inhibition over viral replication) was achieved at addition of extract AH-T-EtOH at its highest concentration and 1 h before inoculating dengue-2 virus strain A15 (VDEN-2 A15). Extract AH-H-ButOH at concentrations of 125 µg/ml and 250 µg/ml presented a slight limitation in the expression of E 1 h after viral inoculation. Extract AH-H-AcEtO at the concentrations used did not show any difference when added before and after inoculation. The immunoperoxidase assay exhibited similar results for the extracts.  Conclusions:  The greatest inhibition of viral replication was obtained with extract AH-H-ButOH at its highest concentration and at both addition times. The enzyme immunoassays applied are useful tools to evaluate Ageratine havanensis extracts with potential antiviral activity.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[ELISA celular]]></kwd>
<kwd lng="es"><![CDATA[inmunoperoxidasa]]></kwd>
<kwd lng="es"><![CDATA[inmunocitología]]></kwd>
<kwd lng="es"><![CDATA[antiviral]]></kwd>
<kwd lng="es"><![CDATA[virus dengue]]></kwd>
<kwd lng="en"><![CDATA[cellular ELISA]]></kwd>
<kwd lng="en"><![CDATA[immunoperoxidase]]></kwd>
<kwd lng="en"><![CDATA[immunocytology]]></kwd>
<kwd lng="en"><![CDATA[antiviral]]></kwd>
<kwd lng="en"><![CDATA[dengue virus]]></kwd>
</kwd-group>
</article-meta>
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