<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0375-0760</journal-id>
<journal-title><![CDATA[Revista Cubana de Medicina Tropical]]></journal-title>
<abbrev-journal-title><![CDATA[Rev Cubana Med Trop]]></abbrev-journal-title>
<issn>0375-0760</issn>
<publisher>
<publisher-name><![CDATA[Centro Nacional de Información de Ciencias Médicas]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0375-07602022000300017</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Estandarización de un ensayo de adhesión de Escherichia coli para el cribado de agentes antibiopelículas]]></article-title>
<article-title xml:lang="en"><![CDATA[Standardization of an Escherichia coli adhesion assay for anti-biofilm agents screening]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Díaz-Reyes]]></surname>
<given-names><![CDATA[Mercyleidi]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Perez-Llanes]]></surname>
<given-names><![CDATA[Mailyn]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Blanco-Hidalgo]]></surname>
<given-names><![CDATA[Odalys]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Espinosa-Castaño]]></surname>
<given-names><![CDATA[Ivette]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Centro Nacional de Sanidad Agropecuaria (CENSA)  ]]></institution>
<addr-line><![CDATA[San José de las Lajas Mayabeque]]></addr-line>
<country>Cuba</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>12</month>
<year>2022</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>12</month>
<year>2022</year>
</pub-date>
<volume>74</volume>
<numero>3</numero>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_arttext&amp;pid=S0375-07602022000300017&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_abstract&amp;pid=S0375-07602022000300017&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_pdf&amp;pid=S0375-07602022000300017&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[RESUMEN  Introducción: Las biopelículas constituyen un factor clave en el desarrollo de enfermedades infecciosas y la resistencia a fármacos para su control. Nuevas estrategias terapéuticas incluyen productos naturales como agentes antibiopelículas. Sin embargo, la comparación de los resultados suele ser difícil debido a la falta de homogeneidad y estandarización en los métodos empleados para estudiar la formación de biopelículas in vitro.  Objetivo: Estandarizar un ensayo de adhesión en microplaca de Escherichia coli para su uso en el cribado de potenciales agentes antibiopelículas.  Métodos: Se utilizó el método de adherencia en microplaca y la tinción con violeta cristal. Se evaluó la influencia de condiciones experimentales como la concentración bacteriana, el medio de cultivo y el tiempo de incubación.  Resultados:  Se identificaron como condiciones óptimas para la formación de biopelículas: el medio Luria Bertani (LB), la concentración bacteriana a 105 UFC/mL y un tiempo de incubación de 24 h.  Conclusiones: Los resultados mostraron que las condiciones de cultivo influyen en la formación de biopelículas. Se determinaron las condiciones de cultivo óptimas para la formación de biopelículas de E. coli, que podrían emplearse en futuros estudios acerca del efecto de productos naturales sobre la inhibición o destrucción de biopelículas.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[ABSTRACT  Introduction: Biofilms are a key factor in the development of infectious diseases and drug resistance for their control. New therapeutic strategies include natural products as anti-biofilm agents. However, comparing results is often difficult due to the lack of homogeneity and standardization of the methods used to study biofilm formation in vitro.  Objective: To standardize an Escherichia coli microplate adhesion assay for potential anti-biofilm agents screening.  Methods: The microplate adhesion method and crystal violet staining were used. The influence of experimental conditions such as bacterial concentration, culture medium, and incubation time were evaluated.  Results: Optimal conditions for biofilm formation included: Luria Bertani (LB) medium, bacterial concentration at 105 CFU/mL, and an incubation time of 24 hours.  Conclusions: The results showed that culture conditions influence biofilm formation. Optimal culture conditions for the formation of E. coli biofilms were determined, which could be used in further studies on the effect of natural products on the inhibition or destruction of biofilms.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[biopelículas]]></kwd>
<kwd lng="es"><![CDATA[Escherichia coli]]></kwd>
<kwd lng="es"><![CDATA[estandarización]]></kwd>
<kwd lng="en"><![CDATA[biofilms]]></kwd>
<kwd lng="en"><![CDATA[Escherichia coli]]></kwd>
<kwd lng="en"><![CDATA[standardization]]></kwd>
</kwd-group>
</article-meta>
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