<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>1010-2752</journal-id>
<journal-title><![CDATA[Revista de Protección Vegetal]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. Protección Veg.]]></abbrev-journal-title>
<issn>1010-2752</issn>
<publisher>
<publisher-name><![CDATA[Centro Nacional de Sanidad Agropecuaria]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S1010-27522019000200002</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[PCR anidado múltiple para detección de fitoplasmas y begomovirus identificados en cultivos de soya en Cuba]]></article-title>
<article-title xml:lang="en"><![CDATA[Nested PCR multiplex assay for detection of phytoplasma and begomoviruses identified in soybean in Cuba]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Leyva Martínez]]></surname>
<given-names><![CDATA[Robert M.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Quiñones Pantoja]]></surname>
<given-names><![CDATA[Madelaine L.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Acosta Peréz]]></surname>
<given-names><![CDATA[Karel]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Piñol Pérez]]></surname>
<given-names><![CDATA[Berta]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Pérez-Álvarez]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Unidad de Extensión, Investigación y Capacitación Agropecuaria de Holguín  ]]></institution>
<addr-line><![CDATA[ Holguín]]></addr-line>
<country>Cuba</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Centro Nacional de Sanidad Agropecuaria  ]]></institution>
<addr-line><![CDATA[San José de Las Lajas Mayabeque]]></addr-line>
<country>Cuba</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,Universidad Vladimir Ilich Lenin  ]]></institution>
<addr-line><![CDATA[ Las Tunas]]></addr-line>
<country>Cuba</country>
</aff>
<aff id="Af4">
<institution><![CDATA[,Universidad Autónoma de Chihuahua Facultad de Ciencias Agrícolas y Forestales Delicias Chihuahua]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Mexico</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>08</month>
<year>2019</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>08</month>
<year>2019</year>
</pub-date>
<volume>34</volume>
<numero>2</numero>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_arttext&amp;pid=S1010-27522019000200002&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_abstract&amp;pid=S1010-27522019000200002&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_pdf&amp;pid=S1010-27522019000200002&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[RESUMEN El objetivo del presente trabajo fue desarrollar un ensayo de PCR múltiple anidado para la detección simultánea de fitoplasmas y los begomovirus identificados en cultivos de soya en Cuba. Se diseñaron dos parejas de cebadores específicos a los begomovirus Tobacco yellow crinkle virus (TbYCV) y Rhynchosia golden mosaic Yucatan virus (RhGMYuV) que se utilizaron, conjuntamente, con dos parejas de cebadores universales para la detección genérica de fitoplasmas bajo condiciones ya establecidas. Se optimizaron los parámetros críticos para el ensayo de PCR anidado para begomovirus que incluyeron: evaluación de la temperatura óptima de alineamiento de los cebadores al molde, sensibilidad analítica y la especificidad de los cebadores. Se optimizaron los parámetros críticos para el ensayo de PCR múltiple anidado; estos fueron: evaluación de diferentes combinaciones de concentraciones de los cebadores diseñados con las condiciones establecidas para nPCR de fitoplasmas, sensibilidad analítica y repetitividad intra e interensayo. Se determinaron los parámetros de desempeño del ensayo de nPCR múltiple. En el ensayo múltiple se amplificaron satisfactoriamente los fragmentos esperados para el TbYCV (988 kb), RhGMYuV (968 kb) y los fitoplasmas (1250 kb), confirmando la detección simultánea, sensible y específica de estos patógenos. Los parámetros analíticos de desempeño del ensayo fueron superiores a 90 %. El ensayo de PCR anidado múltiple desarrollado permite una detección sensible, rápida y de menor costo para la detección de los fitoplasmas y begomovirus que afectan el cultivo de soya en Cuba. Estos resultados son los primeros de este tipo para el país y aportan nuevos elementos metodológicos para la validación de ensayos de detección de patógenos de plantas.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[ABSTRACT The objective of the work was to develop a nested multiplex PCR assay for the simultaneous detection of phytoplasmas and the begomoviruses identified in soybean in Cuba. Two pairs of specific primers to the begomoviruses Tobacco yellow crinkle virus (TbYCV) and Rhynchosia golden mosaic Yucatan virus (RhGMYuV) were designed, and they were used in conjunction with two pairs of universal primers for the generic detection of phytoplasms under established conditions. The evaluation of the optimal temperature for alignment of the primers to the template, the analytical sensitivity, and the specificity of the primers, critical parameters for the nested PCR assay for begomoviruses, were optimized. Critical parameters for the nested multiplex PCR assay, such as the evaluation of different combinations of concentrations of the primers designed with the conditions established for phytoplasm nPCR, the analytical sensitivity, and the intra and inter-assay repeatability, were also optimized. Performance parameters of the multiplex nPCR assay were determined. In the multiple assay, the fragments expected for TbYCV (988 kb), RhGMYuV (968 kb), and phytoplasms (1250 kb) were successfully amplified, confirming the simultaneous, sensitive and specific detection of these pathogens. The analytical parameters of the trial performance were over 90%. The multiple nested PCR assay here developed allows a sensitive, fast and low-cost detection of phytoplasmas sp. and begomoviruses that affect soybean in Cuba. These results are the first of this type for the country and provide new methodological elements for the validation of plant pathogen detection assays.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[begomovirus]]></kwd>
<kwd lng="es"><![CDATA[fitoplasmas]]></kwd>
<kwd lng="es"><![CDATA[PCR anidada múltiple]]></kwd>
<kwd lng="es"><![CDATA[soya]]></kwd>
<kwd lng="en"><![CDATA[begomoviruses]]></kwd>
<kwd lng="en"><![CDATA[phytoplasma]]></kwd>
<kwd lng="en"><![CDATA[multiplex nested PCR]]></kwd>
<kwd lng="en"><![CDATA[soybean]]></kwd>
</kwd-group>
</article-meta>
</front><back>
<ref-list>
<ref id="B1">
<label>1</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Reveles-Torres]]></surname>
<given-names><![CDATA[LR]]></given-names>
</name>
<name>
<surname><![CDATA[Velásquez-Valle]]></surname>
<given-names><![CDATA[VR]]></given-names>
</name>
<name>
<surname><![CDATA[Castillo-Mauricio]]></surname>
<given-names><![CDATA[JA]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Fitoplasmas Otros agentes fitopatógenos. Campo experimental Zacatecas]]></article-title>
<source><![CDATA[Folleto técnico]]></source>
<year>2014</year>
<volume>56</volume>
<page-range>41</page-range><publisher-name><![CDATA[CIRNOC-INIFAP]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B2">
<label>2</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Jamil]]></surname>
<given-names><![CDATA[N]]></given-names>
</name>
<name>
<surname><![CDATA[Rehman]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
<name>
<surname><![CDATA[Hamza]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
<name>
<surname><![CDATA[Hafeez]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
<name>
<surname><![CDATA[Ismail]]></surname>
<given-names><![CDATA[H]]></given-names>
</name>
<name>
<surname><![CDATA[Zubair]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[First Report of Tomato leaf curl New Delhi virus, a Bipartite Begomovirus, Infecting Soybean (Glycine max)]]></article-title>
<source><![CDATA[Plant Disease]]></source>
<year>2017</year>
<volume>101</volume>
<numero>5</numero>
<issue>5</issue>
<page-range>845</page-range></nlm-citation>
</ref>
<ref id="B3">
<label>3</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Ramesh]]></surname>
<given-names><![CDATA[SV]]></given-names>
</name>
<name>
<surname><![CDATA[Chouhan]]></surname>
<given-names><![CDATA[BS]]></given-names>
</name>
<name>
<surname><![CDATA[Gupta]]></surname>
<given-names><![CDATA[GK]]></given-names>
</name>
<name>
<surname><![CDATA[Husain]]></surname>
<given-names><![CDATA[SM]]></given-names>
</name>
<name>
<surname><![CDATA[Chand]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Genomic sequence characterization of Begomovirus infecting soybean and molecular evolutionary genomics of Legume yellow mosaic viruses (LYMVs) Plants Omics Journal]]></article-title>
<source><![CDATA[POJ]]></source>
<year>2017</year>
<volume>10</volume>
<numero>02</numero>
<issue>02</issue>
<page-range>88-96</page-range></nlm-citation>
</ref>
<ref id="B4">
<label>4</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Acosta]]></surname>
<given-names><![CDATA[K]]></given-names>
</name>
<name>
<surname><![CDATA[Silva]]></surname>
<given-names><![CDATA[FN]]></given-names>
</name>
<name>
<surname><![CDATA[Zamora]]></surname>
<given-names><![CDATA[L]]></given-names>
</name>
<name>
<surname><![CDATA[Quiñones]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
<name>
<surname><![CDATA[Martínez]]></surname>
<given-names><![CDATA[R]]></given-names>
</name>
<name>
<surname><![CDATA[Piñol]]></surname>
<given-names><![CDATA[B]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Detection and molecular characterization of an aster yellows group phytoplasma associated with soybean and evidence of two new 16SrI subgroups in Cuba]]></article-title>
<source><![CDATA[Journal of Plant Pathology]]></source>
<year>2015</year>
<volume>97</volume>
<numero>2</numero>
<issue>2</issue>
<page-range>339-44</page-range></nlm-citation>
</ref>
<ref id="B5">
<label>5</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Quiñones]]></surname>
<given-names><![CDATA[ML]]></given-names>
</name>
<name>
<surname><![CDATA[Acosta]]></surname>
<given-names><![CDATA[KI]]></given-names>
</name>
<name>
<surname><![CDATA[Castillo Urquiza]]></surname>
<given-names><![CDATA[G]]></given-names>
</name>
<name>
<surname><![CDATA[Zamora]]></surname>
<given-names><![CDATA[L]]></given-names>
</name>
<name>
<surname><![CDATA[Murilo Zerbini]]></surname>
<given-names><![CDATA[F]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Soya y rábano como nuevos hospedantes de Begomovirus en Cuba]]></article-title>
<source><![CDATA[Rev. Protección Veg.]]></source>
<year>2014</year>
<volume>29</volume>
<numero>3</numero>
<issue>3</issue>
<page-range>235</page-range></nlm-citation>
</ref>
<ref id="B6">
<label>6</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Chang-Sidorchuk]]></surname>
<given-names><![CDATA[L]]></given-names>
</name>
<name>
<surname><![CDATA[González]]></surname>
<given-names><![CDATA[H]]></given-names>
</name>
<name>
<surname><![CDATA[Martínez-Zubiaur]]></surname>
<given-names><![CDATA[Y]]></given-names>
</name>
<name>
<surname><![CDATA[Navas-Castillo]]></surname>
<given-names><![CDATA[J]]></given-names>
</name>
<name>
<surname><![CDATA[Fiallo-Olivé]]></surname>
<given-names><![CDATA[E]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[First report of Rhynchosia golden mosaic Yucatan virus infecting soybean in Cuba]]></article-title>
<source><![CDATA[Journal of Plant Pathology]]></source>
<year>2016</year>
<volume>98</volume>
<page-range>174</page-range></nlm-citation>
</ref>
<ref id="B7">
<label>7</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Lau]]></surname>
<given-names><![CDATA[HY]]></given-names>
</name>
<name>
<surname><![CDATA[Botella]]></surname>
<given-names><![CDATA[JR]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Advanced DNA-Based Point-of-Care Diagnostic Methods for Plant Diseases Detection]]></article-title>
<source><![CDATA[Front. Plant Sci.]]></source>
<year>2017</year>
<page-range>8</page-range></nlm-citation>
</ref>
<ref id="B8">
<label>8</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Bertaccini]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Phytoplasma research between past and future what directions?]]></article-title>
<source><![CDATA[Phytopathogenic Mollicutes]]></source>
<year>2015</year>
<volume>5</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>S1-4</page-range></nlm-citation>
</ref>
<ref id="B9">
<label>9</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Khan]]></surname>
<given-names><![CDATA[J]]></given-names>
</name>
<name>
<surname><![CDATA[Srivastava]]></surname>
<given-names><![CDATA[P]]></given-names>
</name>
<name>
<surname><![CDATA[Singh]]></surname>
<given-names><![CDATA[K]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Efficacy of nested-PCR for the detection of phytoplasma causing spike disease of sandal]]></article-title>
<source><![CDATA[Current Science]]></source>
<year>2004</year>
<volume>86</volume>
<page-range>1530-3</page-range></nlm-citation>
</ref>
<ref id="B10">
<label>10</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Lugo]]></surname>
<given-names><![CDATA[MOY]]></given-names>
</name>
<name>
<surname><![CDATA[Guzmán]]></surname>
<given-names><![CDATA[UR]]></given-names>
</name>
<name>
<surname><![CDATA[García]]></surname>
<given-names><![CDATA[ERS]]></given-names>
</name>
<name>
<surname><![CDATA[León]]></surname>
<given-names><![CDATA[FJ]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Geminivirus transmitidos por mosca blanca (Bemisia tabaci) en tomate del Valle Agrícola de Culiacán, Sinaloa]]></article-title>
<source><![CDATA[Revista Mexicana de Fitopatología]]></source>
<year>2011</year>
<volume>29</volume>
<page-range>109-18</page-range></nlm-citation>
</ref>
<ref id="B11">
<label>11</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Kwon]]></surname>
<given-names><![CDATA[JY]]></given-names>
</name>
<name>
<surname><![CDATA[Hong]]></surname>
<given-names><![CDATA[JS]]></given-names>
</name>
<name>
<surname><![CDATA[Kim]]></surname>
<given-names><![CDATA[MJ]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Simultaneous multiplex PCR detection of seven cucurbit infecting viruses]]></article-title>
<source><![CDATA[J Virol Methods]]></source>
<year>2014</year>
<volume>206</volume>
<page-range>133-9</page-range></nlm-citation>
</ref>
<ref id="B12">
<label>12</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Venkataravanappa]]></surname>
<given-names><![CDATA[V]]></given-names>
</name>
<name>
<surname><![CDATA[Prasanna]]></surname>
<given-names><![CDATA[HC]]></given-names>
</name>
<name>
<surname><![CDATA[Lakshminarayana-Reddy]]></surname>
<given-names><![CDATA[CN]]></given-names>
</name>
<name>
<surname><![CDATA[Krishna-Reddy]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Molecular detection and characterization of phytoplasma in association with begomovirus in eggplant]]></article-title>
<source><![CDATA[Acta virológica]]></source>
<year>2018</year>
<volume>62</volume>
<page-range>246-58</page-range></nlm-citation>
</ref>
<ref id="B13">
<label>13</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Gundersen]]></surname>
<given-names><![CDATA[D]]></given-names>
</name>
<name>
<surname><![CDATA[Lee]]></surname>
<given-names><![CDATA[IM]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Ultrasensitive detection of phytoplasmas by nested PCR assay using two universal primer pairs]]></article-title>
<source><![CDATA[Phytopathology Mediterranean]]></source>
<year>1996</year>
<volume>35</volume>
<page-range>144-51</page-range></nlm-citation>
</ref>
<ref id="B14">
<label>14</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Rychlik]]></surname>
<given-names><![CDATA[W]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Selection of primers for Polimerase Chain Reaction]]></article-title>
<source><![CDATA[Molecular Biotechnology]]></source>
<year>1995</year>
<volume>3</volume>
<page-range>129-34</page-range></nlm-citation>
</ref>
<ref id="B15">
<label>15</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Tamura]]></surname>
<given-names><![CDATA[K]]></given-names>
</name>
<name>
<surname><![CDATA[Stecher]]></surname>
<given-names><![CDATA[G]]></given-names>
</name>
<name>
<surname><![CDATA[Peterson]]></surname>
<given-names><![CDATA[D]]></given-names>
</name>
<name>
<surname><![CDATA[Filipski]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
<name>
<surname><![CDATA[Kumar]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[MEGA6 molecular evolutionary genetics analysis version 6.0]]></article-title>
<source><![CDATA[Molecular biology and evolution]]></source>
<year>2013</year>
<volume>30</volume>
<numero>12</numero>
<issue>12</issue>
<page-range>2725-9</page-range></nlm-citation>
</ref>
<ref id="B16">
<label>16</label><nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Sambrook]]></surname>
<given-names><![CDATA[J]]></given-names>
</name>
<name>
<surname><![CDATA[Fritsch]]></surname>
<given-names><![CDATA[EF]]></given-names>
</name>
<name>
<surname><![CDATA[Maniatis]]></surname>
<given-names><![CDATA[T]]></given-names>
</name>
</person-group>
<source><![CDATA[Molecular cloning]]></source>
<year>1989</year>
<edition>Segunda Edición</edition>
<publisher-name><![CDATA[Cold Spring Harbor Laboratory Press]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B17">
<label>17</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Massart]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
<name>
<surname><![CDATA[Brostaux]]></surname>
<given-names><![CDATA[Y]]></given-names>
</name>
<name>
<surname><![CDATA[Barbarossa]]></surname>
<given-names><![CDATA[L]]></given-names>
</name>
<name>
<surname><![CDATA[Battle]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
<name>
<surname><![CDATA[Cesar]]></surname>
<given-names><![CDATA[V]]></given-names>
</name>
<name>
<surname><![CDATA[Dutercq]]></surname>
<given-names><![CDATA[O]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Inter-laboratory evaluation of two Reverse-transcriptase Polymeric Chain Reaction-based methods for detection of four fruit tree viruses]]></article-title>
<source><![CDATA[Ann. Appl. Biol.]]></source>
<year>2009</year>
<volume>154</volume>
<page-range>133-41</page-range></nlm-citation>
</ref>
<ref id="B18">
<label>18</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Majumder]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
<name>
<surname><![CDATA[Baranwal]]></surname>
<given-names><![CDATA[VK]]></given-names>
</name>
<name>
<surname><![CDATA[Joshi]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Simultaneous detection of Onion yellow dwarf virus and Shallot latent virus in infected leaves and cloves of garlic by duplex RT-PCR]]></article-title>
<source><![CDATA[J. Plant Pathol]]></source>
<year>2008</year>
<volume>90</volume>
<numero>2</numero>
<issue>2</issue>
<page-range>371-4</page-range></nlm-citation>
</ref>
<ref id="B19">
<label>19</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Firrao]]></surname>
<given-names><![CDATA[G]]></given-names>
</name>
<name>
<surname><![CDATA[Garcia-Chapa]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
<name>
<surname><![CDATA[Marzachí]]></surname>
<given-names><![CDATA[C]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Phytoplasmas genetics, diagnosis and relationships with the plants and insects host]]></article-title>
<source><![CDATA[Frontiers in Bioscience]]></source>
<year>2007</year>
<volume>12</volume>
<page-range>1353-75</page-range></nlm-citation>
</ref>
<ref id="B20">
<label>20</label><nlm-citation citation-type="book">
<source><![CDATA[OIE Terrestrial Manual. Principles and methods of validation of diagnostic assays for infectious diseases]]></source>
<year>2013</year>
<publisher-name><![CDATA[World Assembly of Delegates of the OIE]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B21">
<label>21</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Díaz]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
<name>
<surname><![CDATA[Quiñones]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
<name>
<surname><![CDATA[Arana]]></surname>
<given-names><![CDATA[F]]></given-names>
</name>
<name>
<surname><![CDATA[Soto]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
<name>
<surname><![CDATA[Hernández]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Potyvirus características generales, situación de su diagnóstico y determinación de su presencia en cultivo de pimiento en cuba]]></article-title>
<source><![CDATA[Rev. Protección Veg.]]></source>
<year>2010</year>
<volume>25</volume>
<numero>2</numero>
<issue>2</issue>
<page-range>69-79</page-range></nlm-citation>
</ref>
<ref id="B22">
<label>22</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Acosta]]></surname>
<given-names><![CDATA[K]]></given-names>
</name>
<name>
<surname><![CDATA[Martínez]]></surname>
<given-names><![CDATA[Y]]></given-names>
</name>
<name>
<surname><![CDATA[Zamora]]></surname>
<given-names><![CDATA[L]]></given-names>
</name>
<name>
<surname><![CDATA[Fernández]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
<name>
<surname><![CDATA[Santos-Cervantes]]></surname>
<given-names><![CDATA[ME]]></given-names>
</name>
<name>
<surname><![CDATA[Leyva-López]]></surname>
<given-names><![CDATA[NE]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[PCR múltiple anidada para detección de fitoplasmas y rickettsia asociados con los síntomas del cogollo arrepollado (Bts) en papayo]]></article-title>
<source><![CDATA[Rev. Protección Veg.]]></source>
<year>2011</year>
<volume>26</volume>
<numero>3</numero>
<issue>3</issue>
<page-range>156-63</page-range></nlm-citation>
</ref>
<ref id="B23">
<label>23</label><nlm-citation citation-type="">
<source><![CDATA[Manual OIE. Principles of validation of diagnostic assays for infectious diseases]]></source>
<year>2006</year>
</nlm-citation>
</ref>
<ref id="B24">
<label>24</label><nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Galetto]]></surname>
<given-names><![CDATA[L]]></given-names>
</name>
<name>
<surname><![CDATA[Marzachi]]></surname>
<given-names><![CDATA[C]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Real-time PCR Diagnosis and Quantification of Phytoplasmas]]></article-title>
<person-group person-group-type="editor">
<name>
<surname><![CDATA[Weintraub]]></surname>
<given-names><![CDATA[P.G.]]></given-names>
</name>
<name>
<surname><![CDATA[Jones]]></surname>
<given-names><![CDATA[P.]]></given-names>
</name>
</person-group>
<source><![CDATA[Phytoplasmas: Genomes, Plant Hosts and Vectors]]></source>
<year>2010</year>
<page-range>1-18</page-range><publisher-loc><![CDATA[United Kingdom ]]></publisher-loc>
<publisher-name><![CDATA[CAB International]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B25">
<label>25</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Arana]]></surname>
<given-names><![CDATA[F]]></given-names>
</name>
<name>
<surname><![CDATA[Pacheco]]></surname>
<given-names><![CDATA[R]]></given-names>
</name>
<name>
<surname><![CDATA[Díaz De la Osa]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
<name>
<surname><![CDATA[Piñol]]></surname>
<given-names><![CDATA[B]]></given-names>
</name>
<name>
<surname><![CDATA[Quiñones]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Desarrollo de un método para el diagnóstico específico del PepMoV basado en la RT-PCR]]></article-title>
<source><![CDATA[Rev. Protección Veg.]]></source>
<year>2016</year>
<volume>31</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>20-8</page-range></nlm-citation>
</ref>
<ref id="B26">
<label>26</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Naranjo]]></surname>
<given-names><![CDATA[FE]]></given-names>
</name>
<name>
<surname><![CDATA[Martínez]]></surname>
<given-names><![CDATA[ZY]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Avances en el diagnóstico de la marchitez bacteriana (Ralstonia solanacearum) situación actual y perspectivas en Cuba]]></article-title>
<source><![CDATA[Rev. Protección Veg.]]></source>
<year>2013</year>
<volume>28</volume>
<numero>3</numero>
<issue>3</issue>
<page-range>160-70</page-range></nlm-citation>
</ref>
<ref id="B27">
<label>27</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Tao]]></surname>
<given-names><![CDATA[Y]]></given-names>
</name>
<name>
<surname><![CDATA[Man]]></surname>
<given-names><![CDATA[J]]></given-names>
</name>
<name>
<surname><![CDATA[Wu]]></surname>
<given-names><![CDATA[Y]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Development of a multiplex polymerase chain reaction for simultaneous detection of wheat viruses and a phytoplasma in China]]></article-title>
<source><![CDATA[Arch Virol]]></source>
<year>2012</year>
<volume>157</volume>
<page-range>1261-7</page-range></nlm-citation>
</ref>
<ref id="B28">
<label>28</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Swarnalatha]]></surname>
<given-names><![CDATA[P]]></given-names>
</name>
<name>
<surname><![CDATA[Krishna-Reddy]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Duplex PCR for simultaneous detection of Begomovirus and Phytoplasma from naturally infected tomato]]></article-title>
<source><![CDATA[Pest Management in Horticultural Ecosystems]]></source>
<year>2014</year>
<volume>20</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>59-68</page-range></nlm-citation>
</ref>
<ref id="B29">
<label>29</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Biswas]]></surname>
<given-names><![CDATA[C]]></given-names>
</name>
<name>
<surname><![CDATA[Dey]]></surname>
<given-names><![CDATA[P]]></given-names>
</name>
<name>
<surname><![CDATA[Sat pathy]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[A multiplex nested PCR assay for simultaneous detection of Corchorus golden mosaic virus and a phytoplasma in white jute (Corchorus capsularis L)]]></article-title>
<source><![CDATA[Lett. Appl. Microbiol.]]></source>
<year>2013</year>
<volume>56</volume>
<page-range>373-8</page-range></nlm-citation>
</ref>
</ref-list>
</back>
</article>
