Reconocimiento de antígenos lipopolisacarídicos de Pseudomonas aeruginosa por sueros humanos.
Recognises of antigen lipopolysaccharide from Pseudomonas aeruginosa by human sera
Jonatán Hernández1, Jacquelin Alfonso1, Juan F. Almenares2, Aniel Moya1, Jose L. Pérez, Sara C. Esnard1, Armando Cádiz1, Gustavo Sierra1.
1. Instituto Finlay, Centro de Investigación-Producción de Vacunas y Sueros. Ciudad Habana, Cuba.E-mail: jhernandez@finlay.edu.cu; jonatanhr@yahoo.com.
2. Centro de Estudios de Biotecnología Universidad de Oriente, Santiago de Cuba, Cuba.
RESUMEN
]]> En este trabajo se procedió al reconocimiento de lipopolisacáridos de diferentes serotipos de Pseusodomas aeruginosa por sueros humanos. Los lipopolisacáridos fueron aislados por el método Wespthal y Jann. Se utilizaron un total de 104 sueros de pacientes infectados y donantes voluntarios, provenientes de instalaciones hospitalarias y del Banco de Sangre, para el reconocimiento de antígenos lipopolisacarídicos, mediante el ensayo de Dott Blot. Los resultados mostraron que los serotipos de mayor reconocimiento, por los sueros testados, fueron el O11, O13, O16 y el O15, existiendo diferencias significativas entre ellos (p< 0,05).
Palabras claves: Pseudomonas, lipopolisacáridos, serotipos
ABSTRACT
This work presents the recognition of different serotypes of Pseudomonas aeruginosa lipopolysaccharide by human sera. The lipopolysaccharides were purified by the methods of Wespthal and Jann. On the other hand, a total of 104 serums, of infected patients and voluntary donors, coming from different hospitals and from the Blood Bank of the Marianao municipality were used for the recognition of the antigenic lipopolysaccharides, using of dot blot. Our results showed that the serotypes most frequently recognized by the tested serums, were O11, O13, O16 and O15, with significant differences among them (p <0,05).
Keywords: Pseudomonas aeruginosa, lipopolysaccharide, serotypes.
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