<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>1561-3194</journal-id>
<journal-title><![CDATA[Revista de Ciencias Médicas de Pinar del Río]]></journal-title>
<abbrev-journal-title><![CDATA[Rev Ciencias Médicas]]></abbrev-journal-title>
<issn>1561-3194</issn>
<publisher>
<publisher-name><![CDATA[Editorial Ciencias Médicas]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S1561-31942019000200212</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Papel protector del ácido fólico en un biomodelo de intoxicación alcohólica prenatal en ratas Wistar]]></article-title>
<article-title xml:lang="en"><![CDATA[Protective role of folic acid in a biomodel of prenatal alcohol intoxication in Wistar rats]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Gómez García]]></surname>
<given-names><![CDATA[Marcia Leticia]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Linares Guerra]]></surname>
<given-names><![CDATA[Elisa Maritza]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Montier Iglesias]]></surname>
<given-names><![CDATA[Ariel]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Díaz González]]></surname>
<given-names><![CDATA[Odalys]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad de Ciencias Médicas de Pinar del Río  ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Cuba</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Universidad de Pinar del Río Hermanos Saiz Montes de Oca  ]]></institution>
<addr-line><![CDATA[Pinar del río ]]></addr-line>
<country>Cuba</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>04</month>
<year>2019</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>04</month>
<year>2019</year>
</pub-date>
<volume>23</volume>
<numero>2</numero>
<fpage>212</fpage>
<lpage>223</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_arttext&amp;pid=S1561-31942019000200212&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_abstract&amp;pid=S1561-31942019000200212&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_pdf&amp;pid=S1561-31942019000200212&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[RESUMEN  Introducción: el etanol es un agente teratógeno cuyo consumo excesivo representa un importante problema de salud a nivel mundial.  Objetivo: demostrar el papel protector del ácido fólico sobre el Sistema Nervioso Central y las dimensiones craneofaciales, en un biomodelo de intoxicación alcohólica prenatal en ratas Wistar.  Métodos:  estudio experimental con crías de tres grupos de ratas gestantes: sin intoxicación alcohólica; con 5 ml de etanol al 40 % durante la gestación y con 5 ml de etanol al 40 % más 200 µg/día de ácido fólico. Se evaluó en las crías la presencia de meningocele, encefalocele y microcefalia, y se midieron las dimensiones craneofaciales. Se utilizó la comparación de proporciones para muestras independientes, la prueba de Kruskal-Wallis y se estimó una asociación de riesgo estadísticamente significativa para un intervalo de confianza del Odds-Ratio que no contenga la unidad.  Resultados: la suplementación con ácido fólico en ratas gestantes con intoxicación alcohólica, evitó en las crías la aparición de encefalocele, redujo la microcefalia, la disminución del diámetro biparietal y de la distancia entre los globos oculares, sin embrago, no logró evitar totalmente los daños en el Sistema Nervioso Central, ni impedir la disminución del diámetro anteroposterior ni de la distancia poro nasal-oreja.  Conclusión: el biomodelo demostró el efecto tóxico del etanol y la protección del ácido fólico sobre el Sistema Nervioso Central y algunas dimensiones craneofaciales de las crías. Una dosis superior de ácido fólico a la utilizada en el experimento, pudiera ser recomendada para lograr una mayor protección de la descendencia.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[ABSTRACT  Introduction:  ethanol is a teratogenic agent and its excessive consumption represents a major worldwide health problem.  Objective:  to demonstrate the protective role of folic acid on the Central Nervous System and craniofacial dimensions in a biomodel of prenatal alcohol intoxication in Wistar rats.  Methods:  an experimental study with offspring of three groups of pregnant rats: without alcohol intoxication; with 5 ml of 40 % ethanol during gestation and with 5 ml of 40 % ethanol plus 200 µg/day of folic acid. The presence of meningocele, encephalocele and microcephaly was evaluated in the offspring and the craniofacial dimensions were measured. The comparison of proportions for independent samples, the Kruskal-Wallis test and a statistically significant risk association was estimated for an Odds-Ratio confidence interval not containing the unit.  Results:  supplementation with folic acid in pregnant rats with alcohol intoxication prevented the onset of encephalocele of the offspring, reduce the microcephaly, diminution of the biparietal diameter and the distance between the eyeballs, however the damage of the Central Nervous System could not completely avoided, and not prevent the reduction of the anteroposterior diameter or the nasal-ear pore distance.  Conclusion:  the biomodel demonstrated the toxic effect of ethanol and the protection of folic acid on the Central Nervous System and some craniofacial dimensions of the offspring. A higher dose of folic acid, than the one used in the research, could be recommended to achieve a better protection of the offspring.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[ÁCIDO FÓLICO]]></kwd>
<kwd lng="es"><![CDATA[SISTEMA NERVIOSO CENTRAL]]></kwd>
<kwd lng="es"><![CDATA[DIMENSIONES]]></kwd>
<kwd lng="es"><![CDATA[ANOMALÍAS CRANEOFACIALES]]></kwd>
<kwd lng="es"><![CDATA[INTOXICACIÓN ALCOHÓLICA]]></kwd>
<kwd lng="en"><![CDATA[FOLIC ACID]]></kwd>
<kwd lng="en"><![CDATA[CENTRAL NERVOUS SYSTEM]]></kwd>
<kwd lng="en"><![CDATA[DIMENSIONS]]></kwd>
<kwd lng="en"><![CDATA[CRANIOFACIAL ABNORMALITIES]]></kwd>
<kwd lng="en"><![CDATA[ALCOHOLIC INTOXICATION]]></kwd>
</kwd-group>
</article-meta>
</front><back>
<ref-list>
<ref id="B1">
<label>1</label><nlm-citation citation-type="book">
<source><![CDATA[Impactos negativos del alcohol en la salud]]></source>
<year>2016</year>
<publisher-loc><![CDATA[Ginebra ]]></publisher-loc>
<publisher-name><![CDATA[Organización Mundial de la Salud]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B2">
<label>2</label><nlm-citation citation-type="journal">
<collab>Cuba. Centro Nacional de Información de Ciencias Médicas. Biblioteca Médica Nacional</collab>
<article-title xml:lang=""><![CDATA[Alcoholismo. Estadísticas Mundiales]]></article-title>
<source><![CDATA[Factográfico salud]]></source>
<year>2017</year>
<volume>3</volume>
<numero>2</numero>
<issue>2</issue>
</nlm-citation>
</ref>
<ref id="B3">
<label>3</label><nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Niebyl]]></surname>
<given-names><![CDATA[JR]]></given-names>
</name>
<name>
<surname><![CDATA[Weber]]></surname>
<given-names><![CDATA[RJ]]></given-names>
</name>
<name>
<surname><![CDATA[Briggs]]></surname>
<given-names><![CDATA[GG]]></given-names>
</name>
</person-group>
<source><![CDATA[Drugs and environmental agents in pregnancy and lactation: Teratology, epidemiology. En: Gabbe SG, Niebyl JR, Simpson JL, et al. Obstetrics: Normal and Problem Pregnancies]]></source>
<year>2017</year>
<edition>7</edition>
<publisher-loc><![CDATA[PA ]]></publisher-loc>
<publisher-name><![CDATA[Elsevier]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B4">
<label>4</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Hoyme]]></surname>
<given-names><![CDATA[HE]]></given-names>
</name>
<name>
<surname><![CDATA[Kalberg]]></surname>
<given-names><![CDATA[WO]]></given-names>
</name>
<name>
<surname><![CDATA[Elliott]]></surname>
<given-names><![CDATA[AJ]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Updated clinical guidelines for diagnosing fetal alcohol spectrum disorders]]></article-title>
<source><![CDATA[Pediatrics]]></source>
<year>2016</year>
<volume>138</volume>
<numero>2</numero>
<issue>2</issue>
</nlm-citation>
</ref>
<ref id="B5">
<label>5</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[González Armas]]></surname>
<given-names><![CDATA[E]]></given-names>
</name>
<name>
<surname><![CDATA[Cabezas Alfonso]]></surname>
<given-names><![CDATA[HC]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Efectos del ácido fólico sobre variables morfométricas en ratas Wistar recién nacidas]]></article-title>
<source><![CDATA[Rev Ciencias Médicas]]></source>
<year>2015</year>
<volume>19</volume>
<numero>4</numero>
<issue>4</issue>
</nlm-citation>
</ref>
<ref id="B6">
<label>6</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Herrera Batista]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
<name>
<surname><![CDATA[Zumeta Dubé Melvis]]></surname>
<given-names><![CDATA[T]]></given-names>
</name>
<name>
<surname><![CDATA[González Bravo]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Efectos del ácido fólico sobre algunas variables morfométricas del timo de ratas adolescentes con síndrome fetal alcohólco]]></article-title>
<source><![CDATA[Rev Cubana InvestBioméd]]></source>
<year>2012</year>
<volume>31</volume>
<numero>1</numero>
<issue>1</issue>
</nlm-citation>
</ref>
<ref id="B7">
<label>7</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Park]]></surname>
<given-names><![CDATA[J-E]]></given-names>
</name>
<name>
<surname><![CDATA[Choi]]></surname>
<given-names><![CDATA[T]]></given-names>
</name>
<name>
<surname><![CDATA[Ryu]]></surname>
<given-names><![CDATA[Y]]></given-names>
</name>
<name>
<surname><![CDATA[Cho]]></surname>
<given-names><![CDATA[S-I]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[The relationship between mild alcohol consumption andmortality in Koreans: a systematic review and meta-analysis]]></article-title>
<source><![CDATA[BMC Public Health]]></source>
<year>2015</year>
<volume>15</volume>
<numero>918</numero>
<issue>918</issue>
</nlm-citation>
</ref>
<ref id="B8">
<label>8</label><nlm-citation citation-type="book">
<source><![CDATA[Manual de Procedimientos Operacionales de Trabajo]]></source>
<year></year>
<edition>01/0</edition>
<publisher-name><![CDATA[CENPALAB]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B9">
<label>9</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Williams]]></surname>
<given-names><![CDATA[JF]]></given-names>
</name>
<name>
<surname><![CDATA[Smith]]></surname>
<given-names><![CDATA[V]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Fetal alcohol spectrum disorders]]></article-title>
<source><![CDATA[Pediatrics]]></source>
<year>2015</year>
<volume>136</volume>
<numero>5</numero>
<issue>5</issue>
</nlm-citation>
</ref>
<ref id="B10">
<label>10</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Wilhoit]]></surname>
<given-names><![CDATA[LF]]></given-names>
</name>
<name>
<surname><![CDATA[Scott]]></surname>
<given-names><![CDATA[D]]></given-names>
</name>
<name>
<surname><![CDATA[Simecka]]></surname>
<given-names><![CDATA[BA]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Fetal alcohol spectrum disorders: Characteristics, complications, and treatment.Abstract]]></article-title>
<source><![CDATA[Community Mental Health Journal]]></source>
<year>2017</year>
<volume>53</volume>
<numero>6</numero>
<issue>6</issue>
</nlm-citation>
</ref>
<ref id="B11">
<label>11</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Hernández-Fonseca]]></surname>
<given-names><![CDATA[K]]></given-names>
</name>
<name>
<surname><![CDATA[Martinell]]></surname>
<given-names><![CDATA[P]]></given-names>
</name>
<name>
<surname><![CDATA[Reyes-Guzmán]]></surname>
<given-names><![CDATA[C]]></given-names>
</name>
<name>
<surname><![CDATA[Méndez]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[¿La exposición crónica al alcohol induce neurodegeneración en el Sistema Nervioso Central de la rata?]]></article-title>
<source><![CDATA[Salud Ment]]></source>
<year>2015</year>
<volume>38</volume>
<numero>3</numero>
<issue>3</issue>
</nlm-citation>
</ref>
<ref id="B12">
<label>12</label><nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Pérez López]]></surname>
<given-names><![CDATA[JA]]></given-names>
</name>
</person-group>
<source><![CDATA[Tabaco, alcohol y embarazo]]></source>
<year>2016</year>
<publisher-loc><![CDATA[España ]]></publisher-loc>
<publisher-name><![CDATA[Formación Continuada del médico práctico]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B13">
<label>13</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Buitrago]]></surname>
<given-names><![CDATA[MR]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Genética molecular del alcoholismo]]></article-title>
<source><![CDATA[Rev. Fac. Med]]></source>
<year>2015</year>
<volume>63</volume>
<numero>3</numero>
<issue>3</issue>
</nlm-citation>
</ref>
<ref id="B14">
<label>14</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Kendler]]></surname>
<given-names><![CDATA[J]]></given-names>
</name>
<name>
<surname><![CDATA[Jianguang]]></surname>
<given-names><![CDATA[J]]></given-names>
</name>
<name>
<surname><![CDATA[Edwards]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[An Extended Swedish National Adoption Study of Alcohol Use Disorder]]></article-title>
<source><![CDATA[JAMA Psychiatry]]></source>
<year>2015</year>
<volume>72</volume>
<numero>3</numero>
<issue>3</issue>
</nlm-citation>
</ref>
<ref id="B15">
<label>15</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Wass]]></surname>
<given-names><![CDATA[T]]></given-names>
</name>
<name>
<surname><![CDATA[Chudley]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Relationships between Head Circumference, Brain Volume and Cognition in Children with prenatal alcohol exposure]]></article-title>
<source><![CDATA[PlosOne]]></source>
<year>2016</year>
<volume>11</volume>
<numero>2</numero>
<issue>2</issue>
</nlm-citation>
</ref>
</ref-list>
</back>
</article>
