<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>2079-3480</journal-id>
<journal-title><![CDATA[Cuban Journal of Agricultural Science]]></journal-title>
<abbrev-journal-title><![CDATA[Cuban J. Agric. Sci.]]></abbrev-journal-title>
<issn>2079-3480</issn>
<publisher>
<publisher-name><![CDATA[Editorial del Instituto de Ciencia Animal]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S2079-34802020000100055</article-id>
<title-group>
<article-title xml:lang="en"><![CDATA[Production of lignocellulases enzymes from Trichoderma viride M5-2 in wheat bran (Triticum aestivum) and purification of their laccases]]></article-title>
<article-title xml:lang="es"><![CDATA[Producción de enzimas lignocelulasas de Trichoderma viride M5-2 en salvado de trigo (Triticum aestivum) y purificación de sus lacasas]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Valiño Cabrera]]></surname>
<given-names><![CDATA[Elaine Cristina]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Alberto Vázquez]]></surname>
<given-names><![CDATA[Maryen]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Dustet Mendoza]]></surname>
<given-names><![CDATA[J. C.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Albelo Dorta]]></surname>
<given-names><![CDATA[Nereyda]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Instituto de Ciencia Animal (ICA)  ]]></institution>
<addr-line><![CDATA[San José de las Lajas Mayabeque]]></addr-line>
<country>Cuba</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Universidad Tecnológica de la Habana &#8220;José Antonio Echeverría&#8221; (CUJAE) Facultad de Ingeniería Química ]]></institution>
<addr-line><![CDATA[ La Habana]]></addr-line>
<country>Cuba</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>03</month>
<year>2020</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>03</month>
<year>2020</year>
</pub-date>
<volume>54</volume>
<numero>1</numero>
<fpage>55</fpage>
<lpage>66</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_arttext&amp;pid=S2079-34802020000100055&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_abstract&amp;pid=S2079-34802020000100055&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_pdf&amp;pid=S2079-34802020000100055&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract The present study describes the production of lignocellulases enzymes from Trichoderma viride M5-2 in wheat bran (Triticum aestivum) and the purification of their laccases. Fermentation process was determined in wheat bran during 7 days and samples were taken every 24h. The enzymatic assay (endo, exo &#946;1, 4-glucanase, xylanases and laccases) was performed. Laccase activity was determined with a spectrophotometer, by syringaldazine oxidation under aerobic conditions and lignin peroxidase was determined by oxidative dimerization dependent on H2O2 of 2.4-dichlorophenol. The enzyme crude was concentrated by membrane filtration with nitrogen stream and purified by ion exchange chromatography with DEAE matrix. Enzyme yield and purification parameters were measured. With the fermentation conditions in wheat bran, a sustained increase in the production of endo &#946;1,4 glucanase and xylanases was obtained after 72 h and exo &#946;1, 4 glucanase at 48 h and laccase enzymatic activity was checked at 24 h and lignin peroxidase after 48h of fermentation. The fungus T. viride M5-2 reached its maximum production of laccases after two days of fermentation and through the proposed purification system, an enzymatic product with a purification factor of 12 was obtained, without affecting the enzyme yield. It is concluded that the T viride strain has the capacity to produce the lignocellulolytic enzyme complex in wheat bran. The separation method used to purify laccase enzymes is effective. It is recommended to add successive steps of purification depending on the degree of purity.]]></p></abstract>
<abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen Esta investigación describe la producción de enzimas lignocelulasas del Trichoderma viride M5-2 en salvado de trigo (Triticum aestivum) y la purificación de sus lacasas. El proceso de fermentación se realizó en el salvado de trigo durante 7 días y se tomaron muestras cada 24 h. Se realizó el ensayo enzimático (endo, exo &#946;1,4 glucanasa, xilanasas y lacasas). La actividad de la lacasa se determinó por espectrofotometría, por oxidación de siringaldazina en condiciones aeróbicas, y la lignina peroxidasa por dimerización oxidativa dependiente de H2O2 de 2,4-diclorofenol. El crudo enzimático se concentró por filtración de la membrana con un flujo de nitrógeno y se purifico a través de cromatografía de intercambio iónico con matriz de DEAE. Se midieron los indicadores de purificación y el rendimiento de la enzima. Con las condiciones de fermentación del salvado de trigo se obtuvo un aumento continuo de la producción de endo &#946; 1,4 glucanasa and xilanasas después de 72 h y de exo &#946;1, 4 glucanasa a las 48 h. Además, la actividad enzimática de la lacasa se supervisó a las 24h y la lignina peroxidasa tras 48 h d fermentación. El hongo T. viride M5-2 alcanzó su máxima producción de lacasas después de 2 días de fermentación y a través del sistema propuesto de purificación, se obtuvo un producto enzimático con factor de purificación de 12, sin afectar el rendimiento enzimático. Se concluye que la cepa de T. viride tiene la capacidad de producir un complejo de enzimas ligncelulticas en el salvado de trigo. El método de separación utilizado para purificar las enzimas lacasas es efectivo. Se recomienda añadir etapas adicionales de purificación, en dependencia del grado de pureza.]]></p></abstract>
<kwd-group>
<kwd lng="en"><![CDATA[ligninase]]></kwd>
<kwd lng="en"><![CDATA[fungi]]></kwd>
<kwd lng="en"><![CDATA[lignin]]></kwd>
<kwd lng="en"><![CDATA[cellulases]]></kwd>
<kwd lng="en"><![CDATA[xylanases]]></kwd>
<kwd lng="es"><![CDATA[ligninasa]]></kwd>
<kwd lng="es"><![CDATA[hongos]]></kwd>
<kwd lng="es"><![CDATA[lignina]]></kwd>
<kwd lng="es"><![CDATA[celulasas]]></kwd>
<kwd lng="es"><![CDATA[xilanasas]]></kwd>
</kwd-group>
</article-meta>
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<source><![CDATA[Biomass and Bioenergy]]></source>
<year>2018</year>
<volume>112</volume>
<page-range>93-8</page-range></nlm-citation>
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</article>
