<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>2221-2450</journal-id>
<journal-title><![CDATA[Revista CENIC Ciencias Biológicas]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. CENIC Cienc. Biol]]></abbrev-journal-title>
<issn>2221-2450</issn>
<publisher>
<publisher-name><![CDATA[Centro Nacional de Investigaciones Científicas]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S2221-24502021000300259</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Validación de la microscopía de fluorescencia LED para el diagnóstico de tuberculosis en Cuba]]></article-title>
<article-title xml:lang="en"><![CDATA[Validation of LED fluorescence microscopy for tuberculosis diagnosis in Cuba]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Martínez Romero]]></surname>
<given-names><![CDATA[María R.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Pedrera Pozo]]></surname>
<given-names><![CDATA[Nancy]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[García León]]></surname>
<given-names><![CDATA[Grechen C.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Sardiñas Aragón]]></surname>
<given-names><![CDATA[Misleidis]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Mederos Cuervo]]></surname>
<given-names><![CDATA[Lilian M]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Díaz Rodríguez]]></surname>
<given-names><![CDATA[Raúl]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Instituto de Medicina Tropical &#8220;Pedro Kourì&#8221; Laboratorio Nacional de Referencia e Investigaciones en Tuberculosis, Lepra y Micobacterias ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Cuba</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>12</month>
<year>2021</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>12</month>
<year>2021</year>
</pub-date>
<volume>52</volume>
<numero>3</numero>
<fpage>259</fpage>
<lpage>266</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_arttext&amp;pid=S2221-24502021000300259&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_abstract&amp;pid=S2221-24502021000300259&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_pdf&amp;pid=S2221-24502021000300259&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[RESUMEN La microscopía de fluorescencia LED (MF LED) ha sido recomendada por la Organización Mundial de la Salud para el diagnóstico de la tuberculosis (TB). El objetivo fue validar esta técnica en el Laboratorio Nacional de Referencia de Tuberculosis, Lepra y Micobacterias (LNRITBLM). Se realizó un estudio de validación de la MF LED en el LNRITBLM del Instituto &#8220;Pedro Kourí&#8221;, de febrero-julio de 2018. Se incluyeron un total de 98 láminas: 49 frotis que tuvieron cultivo positivo a micobacterias y 49 láminas con cultivo negativo. Se compararon los resultados de la MF LED con los de la microscopía de campo brillante (MCB) y microscopía de fluorescencia convencional (MFC). Con la MF LED y MFC se identificaron 14 láminas positivas que habían sido negativas por MCB, incrementándose la positividad a 13,3%. Por ambas técnicas fluorescentes se detectaron más frotis (18/98) con escasos bacilos ácido alcohol resistentes que por la MCB (8/98). La concordancia entre MF LED y MCB se consideró buena (k= 0,6773) y entre MF LED y MFC muy buena (k= 0,8711). La sensibilidad de la MF LED fue mayor (77,55%) que la MCB (48,98%). Se confirmó la mayor sensibilidad de la MF LED, validando esta técnica como una alternativa a la tinción de Zielh Neelsen en el LNRITBLM. Su implementación en Cuba sería de gran utilidad en la detección y seguimiento del tratamiento de los casos, así como el manejo de los pacientes con sospecha de TB.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[ABSTRACT LED fluorescence microscopy (MF LED) has been recommended by the World Health Organization for tuberculosis (TB) diagnosis. The aim was to validate this technique in the National Reference Laboratory for Tuberculosis, Leprosy and Mycobacteria (NRLTBLM). A validation study of the MF LED was carried out at the NRLTBLM of the &#8220;Pedro Kourí&#8221; Institute, from February-July 2018. A total of 98 slides were included: 49 smears that had a positive culture for mycobacteria and 49 slides with a negative culture. The results of the LED FM were compared with bright field microscopy (BFM) and conventional fluorescence microscopy (CFM). With the LED FM and CFM, 14 positive smears were identified that had been negative by BFM, the positivity rate being increased to 13,3%. By both fluorescent techniques, more smears (18/98) were detected with scanty acid fast bacilli than the BFM (8/98). The agreement between MF LED and BFM was considered good (k = 0,6773) and between MF LED and CFM very good (k = 0,8711). The sensitivity of the MF LED was higher (77,55%) than the BFM (48,98%). The higher sensitivity of the MF LED was confirmed, validating this technique as an alternative to Zielh Neelsen staining in the NRLTBLM. Its implementation in Cuba would be very useful in detecting and monitoring the follow up cases, as well as the management of patients with suspected TB.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[microscopia de fluorescencia LED]]></kwd>
<kwd lng="es"><![CDATA[micobacterias]]></kwd>
<kwd lng="es"><![CDATA[microscopia de campo brillante]]></kwd>
<kwd lng="es"><![CDATA[cultivo]]></kwd>
<kwd lng="en"><![CDATA[LED fluorescence microscopy]]></kwd>
<kwd lng="en"><![CDATA[mycobacteria]]></kwd>
<kwd lng="en"><![CDATA[bright field microscopy]]></kwd>
<kwd lng="en"><![CDATA[culture]]></kwd>
</kwd-group>
</article-meta>
</front><back>
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