<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>2224-5421</journal-id>
<journal-title><![CDATA[Revista Cubana de Química]]></journal-title>
<abbrev-journal-title><![CDATA[Rev Cub Quim]]></abbrev-journal-title>
<issn>2224-5421</issn>
<publisher>
<publisher-name><![CDATA[Ediciones UO, Universidad de Oriente]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S2224-54212020000200172</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Modelación por homología de la tirosinasa hipotética en pleurotus ostreatus PC15]]></article-title>
<article-title xml:lang="en"><![CDATA[Modelling by homology of hypothetical tyrosinase in pleurotus ostreatus PC15]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Benavides-Oro]]></surname>
<given-names><![CDATA[Ianna Y.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Peña-López]]></surname>
<given-names><![CDATA[Javier]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Cutiño-Viñals]]></surname>
<given-names><![CDATA[Lisandra M.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Contreras-Quesada]]></surname>
<given-names><![CDATA[Armando]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Morris-Quevedo]]></surname>
<given-names><![CDATA[Humberto J.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad de Oriente Facultad de Ciencias Naturales y Exactas Departamento de Biología y Geografía]]></institution>
<addr-line><![CDATA[Santiago de Cuba ]]></addr-line>
<country>Cuba</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Universidad de Oriente Facultad de Ciencias Naturales y Exactas Centro de Estudios de Biotecnología Industrial]]></institution>
<addr-line><![CDATA[Santiago de Cuba ]]></addr-line>
<country>Cuba</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>08</month>
<year>2020</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>08</month>
<year>2020</year>
</pub-date>
<volume>32</volume>
<numero>2</numero>
<fpage>172</fpage>
<lpage>194</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_arttext&amp;pid=S2224-54212020000200172&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_abstract&amp;pid=S2224-54212020000200172&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_pdf&amp;pid=S2224-54212020000200172&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[RESUMEN La enzima tirosinasa se relaciona con el oscurecimiento enzimático de los hongos, considerándose la causa principal de la disminución de su valor comercial. Se seleccionó como plantilla la estructura 4j3p A de Aspergillus oryzae, por su similitud con la secuencia de la proteína de Pleurotus ostreatus PC15. Los modelos fueron construidos utilizando los servidores Swiss-model, I-TASSER y Phyre2 y validados por medio del diagrama de Ramachandran suministrado por Procheck, los servidores Verify3D y ProSA. Se obtuvieron tres modelos tridimensionales para la enzima tirosinasa hipotética de Pleurotus ostreatus PC15 validados por diferentes estrategias computacionales. El modelo Tyr Swiss resultó el mejor con un 85,5 % de los residuos de aminoácidos bajo la región favorecida, y un z-score de -7,15 en ProSA. El valor de Verify 3D de 80,73 % indicó que el entorno del perfil del modelo es apropiado. Los modelos obtenidos podrían ser útiles para la búsqueda de nuevos inhibidores de la enzima con aplicaciones industriales.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[ABSTRACT The enzyme tyrosinase is related to the enzymatic darkening of fungi, and it is considered as the main cause of the decrease in the commercial value. The 4j3p A structure of Aspergillus oryzae was selected as template for his similitude with the sequence of the hypothetical tyrosinase from Pleurotus ostreatus PC15. The models were built using the Swiss-model, I-TASSER and Phyre2 servers, and validated by means of the Ramachandran diagram supplied by Procheck, the Verify3D and ProSA servers. Three 3D models were obtained for the hypothetical tyrosinase enzyme of Pleurotus ostreatus PC15, validated by different computational strategies. Tyr Swiss was the best model with 85,5% of the amino acid residues under the favored region, and a z-score of -7,15 in ProSA. The Verify 3D value of 80.73%, indicated that the environment of the model profile was appropriated. The obtained models may be useful for discovery new enzyme inhibitors with industrial applications.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[homología estructural]]></kwd>
<kwd lng="es"><![CDATA[diagrama de Ramachandran]]></kwd>
<kwd lng="es"><![CDATA[oscurecimiento enzimático]]></kwd>
<kwd lng="es"><![CDATA[in silico]]></kwd>
<kwd lng="en"><![CDATA[structural homology]]></kwd>
<kwd lng="en"><![CDATA[Ramachandran diagram]]></kwd>
<kwd lng="en"><![CDATA[enzymatic darkening]]></kwd>
<kwd lng="en"><![CDATA[in silico]]></kwd>
</kwd-group>
</article-meta>
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