<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>2224-5421</journal-id>
<journal-title><![CDATA[Revista Cubana de Química]]></journal-title>
<abbrev-journal-title><![CDATA[Rev Cub Quim]]></abbrev-journal-title>
<issn>2224-5421</issn>
<publisher>
<publisher-name><![CDATA[Ediciones UO, Universidad de Oriente]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S2224-54212020000300527</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Evaluación de la actividad anti-angiogénica del metil jasmonato en membrana corioalantoica de embriones de pollos mediante análisis de imágenes digitales]]></article-title>
<article-title xml:lang="en"><![CDATA[Evaluation of the anti-angiogenic activity of methyl jasmonate in chorioallantoic membrane of chicken embryos by means of digital image analysis]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Escobedo-Nicot]]></surname>
<given-names><![CDATA[Miriela Milagros]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Mojícar-Martínez]]></surname>
<given-names><![CDATA[Guillermo]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Delgado-Font]]></surname>
<given-names><![CDATA[Wilkie Ernesto]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Cruz]]></surname>
<given-names><![CDATA[Leandro Ticlia de la]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Ferreira-Gomes]]></surname>
<given-names><![CDATA[Ligia]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad de Oriente Facultad de Ciencias Naturales y Exactas ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Cuba</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Universidade de São Paulo Instituto Oceanográfico ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Brazil</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,Universidade de São Paulo Faculdade de Ciências Farmacêuticas e Instituto de Física Laboratório de Microrreologia e Fisiologia Molecular]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Brazil</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>12</month>
<year>2020</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>12</month>
<year>2020</year>
</pub-date>
<volume>32</volume>
<numero>3</numero>
<fpage>527</fpage>
<lpage>542</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_arttext&amp;pid=S2224-54212020000300527&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_abstract&amp;pid=S2224-54212020000300527&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_pdf&amp;pid=S2224-54212020000300527&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[RESUMEN Se presenta un estudio para medir el efecto del Metil Jasmonato (MJ) sobre la angiogénesis en muestras de membrana corioalantoica (CAM) de embriones de pollos, usando un sistema de análisis de imágenes. Se consideran como características: puntos terminales, puntos de bifurcación, densidad de área, longitud vascular y dimensión fractal, entre otras. Además, se incluye la clasificación de los vasos sanguíneos usando las taxonomías de Strahler y Tokunaga, lo que permite clasificar segmentos de estructuras jerárquicas. Para la evaluación de los resultados se emplearon muestras control y otras tratadas con MJ en agua y liposomas en tres concentraciones 7.5, 15 y 30 µM. La tendencia anti-angiogénica se corroboró en el ambiente de liposomas considerando la disminución de los valores cuantitativos de todas las características valoradas con respecto a las muestras control. Los resultados obtenidos concuerdan con datos de la literatura respecto a la mejor biodisponibilidad asociada con el vehículo lipídico.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[ABSTRACT A study is presented to measure the effect of Methyl Jasmonate (MJ) on angiogenesis in chorioallantoic membrane (CAM) samples from chicken embryos, using an imaging analysis software. The characteristics considered are: terminal points, bifurcation points, area density, vascular length and fractal dimension, among others. In addition, the classification of blood vessels using Strahler and Tokunaga taxonomies is included, which allows classifying segments of hierarchical structures. For the evaluation of the results, control samples and others treated with MJ in water and liposomes are used in three concentrations 7.5, 15 and 30 µM. The anti-angiogenic tendency was corroborated in the environment of liposomes considering the decrease of the quantitative values of all the evaluated characteristics with regard to the control samples. The results obtained agree with data from literature regarding the best bioavailability associated to the lipid vehicle.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[cuantificación]]></kwd>
<kwd lng="es"><![CDATA[angiogénesis]]></kwd>
<kwd lng="es"><![CDATA[metil jasmonato]]></kwd>
<kwd lng="es"><![CDATA[CAM]]></kwd>
<kwd lng="en"><![CDATA[quantification]]></kwd>
<kwd lng="en"><![CDATA[angiogenesis]]></kwd>
<kwd lng="en"><![CDATA[methyl jasmonate]]></kwd>
<kwd lng="en"><![CDATA[CAM]]></kwd>
</kwd-group>
</article-meta>
</front><back>
<ref-list>
<ref id="B1">
<label>1</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[VIALLARD]]></surname>
<given-names><![CDATA[C.]]></given-names>
</name>
<name>
<surname><![CDATA[LARRIVÉE]]></surname>
<given-names><![CDATA[B.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[&#8220;Tumor angiogenesis and vascular normalization: alternative therapeutic targets]]></article-title>
<source><![CDATA[Angiogenesis]]></source>
<year>2017</year>
<volume>20</volume>
<numero>4</numero>
<issue>4</issue>
<page-range>409-26</page-range></nlm-citation>
</ref>
<ref id="B2">
<label>2</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[NOWAK-SLIWINSKA]]></surname>
<given-names><![CDATA[P.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Consensus guidelines for the use and interpretation of angiogenesis assays]]></article-title>
<source><![CDATA[Angiogenesis]]></source>
<year>2018</year>
<volume>21</volume>
<numero>3</numero>
<issue>3</issue>
<page-range>425-532</page-range></nlm-citation>
</ref>
<ref id="B3">
<label>3</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[SHOEIBI]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<name>
<surname><![CDATA[MOZDZIAK]]></surname>
<given-names><![CDATA[P.]]></given-names>
</name>
<name>
<surname><![CDATA[MOHAMMADI]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Important signals regulating coronary artery angiogenesis]]></article-title>
<source><![CDATA[Microvascular research]]></source>
<year>2018</year>
<volume>117</volume>
<page-range>1-9</page-range></nlm-citation>
</ref>
<ref id="B4">
<label>4</label><nlm-citation citation-type="">
<person-group person-group-type="author">
<name>
<surname><![CDATA[RIBATTI]]></surname>
<given-names><![CDATA[D.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[The Fundamental Contribution of Judah Folkman in the Setting of Angiogenesis Assays]]></article-title>
<person-group person-group-type="editor">
<name>
<surname><![CDATA[Ribatti]]></surname>
<given-names><![CDATA[D.]]></given-names>
</name>
</person-group>
<source><![CDATA[Vascular Morphogenesis: Methods in Molecular Biology. Humana]]></source>
<year>2020</year>
<volume>2206</volume>
<page-range>15-25</page-range></nlm-citation>
</ref>
<ref id="B5">
<label>5</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[RIBATTI]]></surname>
<given-names><![CDATA[D.]]></given-names>
</name>
<name>
<surname><![CDATA[ANNESE]]></surname>
<given-names><![CDATA[T.]]></given-names>
</name>
<name>
<surname><![CDATA[TAMMA]]></surname>
<given-names><![CDATA[R.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[The use of the chick embryo CAM assay in the study of angiogenic activiy of biomaterials]]></article-title>
<source><![CDATA[Microvascular Research]]></source>
<year>2020</year>
<volume>131</volume>
<page-range>104026</page-range></nlm-citation>
</ref>
<ref id="B6">
<label>6</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[NOWAK&#8208;SLIWINSKA]]></surname>
<given-names><![CDATA[P.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Angiostatic kinase inhibitors to sustain photodynamic angio-occlusion]]></article-title>
<source><![CDATA[Journal of Cellular and Molecular Medicine]]></source>
<year>2012</year>
<volume>16</volume>
<numero>7</numero>
<issue>7</issue>
<page-range>1553-62</page-range></nlm-citation>
</ref>
<ref id="B7">
<label>7</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[ÖZCETIN]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
<name>
<surname><![CDATA[AIGNER]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
<name>
<surname><![CDATA[BAKOWSKY]]></surname>
<given-names><![CDATA[U.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[A chorioallantoic membrane model for the determination of anti-angiogenic effects of imatinib]]></article-title>
<source><![CDATA[European Journal of Pharmaceutics and Biopharmaceutics]]></source>
<year>2013</year>
<volume>85</volume>
<numero>3</numero>
<issue>3</issue>
<page-range>711-5</page-range></nlm-citation>
</ref>
<ref id="B8">
<label>8</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[SHI]]></surname>
<given-names><![CDATA[P.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Automated computational framework of blood vessel quantification in chick chorioallantoic membrane angiogenesis]]></article-title>
<source><![CDATA[Journal of Biomedical Optics]]></source>
<year>2014</year>
<volume>19</volume>
<numero>10</numero>
<issue>10</issue>
<page-range>106005</page-range></nlm-citation>
</ref>
<ref id="B9">
<label>9</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[ZAHID]]></surname>
<given-names><![CDATA[H. M. S.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Quantification of angiogenic characteristics of Naproxen sodium through chorioallantoic membrane assay]]></article-title>
<source><![CDATA[Biomedical Research and Therapy]]></source>
<year>2017</year>
<volume>4</volume>
<numero>5</numero>
<issue>5</issue>
<page-range>1281-94</page-range></nlm-citation>
</ref>
<ref id="B10">
<label>10</label><nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[PEREIRA LOPES]]></surname>
<given-names><![CDATA[J. E. F.]]></given-names>
</name>
</person-group>
<source><![CDATA[Efeito antiangiogênico do metil jasmonato, puro ou nanocarreado, um novo mecanismo para sua ação antineoplásica e antimetastática]]></source>
<year>2009</year>
<publisher-loc><![CDATA[Brasil ]]></publisher-loc>
<publisher-name><![CDATA[Universidade de São Paulo]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B11">
<label>11</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[PEREIRA LOPES]]></surname>
<given-names><![CDATA[J. E. F.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[In vivo anti-angiogenic effects further support the promise of the antineoplasic activity of methyl jasmonate]]></article-title>
<source><![CDATA[Brazilian Journal of Biology]]></source>
<year>2010</year>
<volume>70</volume>
<numero>2</numero>
<issue>2</issue>
<page-range>443-9</page-range></nlm-citation>
</ref>
<ref id="B12">
<label>12</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[KHAN]]></surname>
<given-names><![CDATA[M. A.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Characterization and anti-proliferative activity of curcumin loaded chitosan nanoparticles in cervical cancer]]></article-title>
<source><![CDATA[International journal of biological macromolecules]]></source>
<year>2016</year>
<volume>93</volume>
<page-range>242-53</page-range></nlm-citation>
</ref>
<ref id="B13">
<label>13</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[GUERRA]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Sprouting Angiogenesis: A Numerical Approach with Experimental Validation]]></article-title>
<source><![CDATA[Annals of Biomedical Engineering]]></source>
<year>2020</year>
<page-range>1-14</page-range></nlm-citation>
</ref>
<ref id="B14">
<label>14</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[MILI&#268;I&#262;]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Box-counting dimensions of generalised fractal nests]]></article-title>
<source><![CDATA[Chaos, Solitons &amp; Fractals]]></source>
<year>2018</year>
<volume>113</volume>
<page-range>125-34</page-range></nlm-citation>
</ref>
<ref id="B15">
<label>15</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[CHEN]]></surname>
<given-names><![CDATA[Y]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Fractals and fractal dimension of systems of blood vessels: An analogy between artery trees, river networks, and urban hierarchies]]></article-title>
<source><![CDATA[Fractal Geometry and Nonlinear Analysis in Medicine and Biology]]></source>
<year>2015</year>
<volume>1</volume>
<numero>2</numero>
<issue>2</issue>
<page-range>26-32</page-range></nlm-citation>
</ref>
<ref id="B16">
<label>16</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[TAKAKI]]></surname>
<given-names><![CDATA[R]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Branching Structures in Nature and Human Societies]]></article-title>
<source><![CDATA[Form]]></source>
<year>2016</year>
<volume>31</volume>
<page-range>S3-S15</page-range></nlm-citation>
</ref>
<ref id="B17">
<label>17</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[KOPYLOVA]]></surname>
<given-names><![CDATA[V. S.]]></given-names>
</name>
<name>
<surname><![CDATA[BORONOVSKIY]]></surname>
<given-names><![CDATA[S. E.]]></given-names>
</name>
<name>
<surname><![CDATA[NARTSISSOV]]></surname>
<given-names><![CDATA[Y. R]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Fundamental principles of vascular network topology]]></article-title>
<source><![CDATA[Biochemical Society Transactions]]></source>
<year>2017</year>
<volume>45</volume>
<numero>3</numero>
<issue>3</issue>
<page-range>839-44</page-range></nlm-citation>
</ref>
<ref id="B18">
<label>18</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[AKITA]]></surname>
<given-names><![CDATA[D.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Experimental models for Murray&#8217;s law]]></article-title>
<source><![CDATA[Journal of Physics D: Applied Physics]]></source>
<year>2016</year>
<volume>50</volume>
<numero>2</numero>
<issue>2</issue>
<page-range>024001</page-range></nlm-citation>
</ref>
</ref-list>
</back>
</article>
