<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>2224-5421</journal-id>
<journal-title><![CDATA[Revista Cubana de Química]]></journal-title>
<abbrev-journal-title><![CDATA[Rev Cub Quim]]></abbrev-journal-title>
<issn>2224-5421</issn>
<publisher>
<publisher-name><![CDATA[Ediciones UO, Universidad de Oriente]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S2224-54212022000300445</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Producción simultánea de ramnolípidos y polihidroxialcanoatos por Pseudomonas aeruginosa ORA9]]></article-title>
<article-title xml:lang="en"><![CDATA[Simultaneous production of ramnolipids and polyhydroxyalkanoates by Pseudomonas aeruginosa ORA9]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Rodríguez-Gámez]]></surname>
<given-names><![CDATA[Odalys]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Ábalos-Rodríguez]]></surname>
<given-names><![CDATA[Arelis]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Cabrera-Gómez]]></surname>
<given-names><![CDATA[José Gregorio]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad de Oriente Facultad de Ciencias Naturales y Exactas Centro de Estudios de Biotecnología Industrial]]></institution>
<addr-line><![CDATA[Santiago de Cuba ]]></addr-line>
<country>Cuba</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Universidad de Sao Paulo Departamento de Microbiología Instituto de Ciencias Biomédicas II]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Brasil</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>12</month>
<year>2022</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>12</month>
<year>2022</year>
</pub-date>
<volume>34</volume>
<numero>3</numero>
<fpage>445</fpage>
<lpage>461</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_arttext&amp;pid=S2224-54212022000300445&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_abstract&amp;pid=S2224-54212022000300445&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_pdf&amp;pid=S2224-54212022000300445&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[RESUMEN En este trabajo se estudió la cinética de la producción simultánea de ramnolípidos y polihidroxialcanoatos a partir de Pseudomonas aeruginosa ORA9. Se determinaron los parámetros cinéticos, velocidad específica de crecimiento celular (µx), velocidad volumétrica (rp) y específica de formación de producto (qP), y el rendimiento del producto respeto a la biomasa formada (YP/X). Los resultados mostraron que la producción de polihidroxialcanoatos tuvo una acumulación máxima de 22 % (1,14 g L-1) en la biomasa, durante la fase exponencial del crecimiento microbiano. Por el contrario, el mayor rendimiento de ramnolípidos se logra al finalizar el cultivo (YP/X = 1,024) con un incremento de las variables cinéticas evaluadas a partir de las 18 h, coincidiendo con el inicio de la fase estacionaria del crecimiento microbiano. Se comprobó que, en las condiciones ensayadas, el tiempo de cultivo es un factor importante que debe optimizarse para obtener concentraciones adecuadas de ambos productos.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[ABSTRACT In this work, the kinetics of the simultaneous production of rhamnolipids and polyhydroxyalkanoates from Pseudomonas aeruginosa ORA9 was studied. The kinetic parameters specific cell growth rate (µx), volumetric rate (rp) and specific rate of product formation (qP), and the yield of the product with respect to the biomass formed (YP/X) were determined. The results showed that polyhydroxyalkanoates production had a maximum accumulation of 22 % (1,14 g L-1) in the biomass, during the exponential phase of microbial growth. On the contrary, the highest yield of rhamnolipids is achieved at the end of the culture (YP/X = 1,024) with an increase in the kinetic variables evaluated after 18 hours, coinciding with the beginning of the stationary phase of microbial growth. It was found that, under the conditions tested, the culture time is an important factor that must be optimized to obtain adequate concentrations of both products.at the end of culture.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[ramnolípidos]]></kwd>
<kwd lng="es"><![CDATA[polihidroxialcanoatos]]></kwd>
<kwd lng="es"><![CDATA[pseudomonas]]></kwd>
<kwd lng="es"><![CDATA[cinética]]></kwd>
<kwd lng="en"><![CDATA[rhamnolipids]]></kwd>
<kwd lng="en"><![CDATA[polyhydroxyalkanoates]]></kwd>
<kwd lng="en"><![CDATA[pseudomonas]]></kwd>
<kwd lng="en"><![CDATA[kinetic]]></kwd>
</kwd-group>
</article-meta>
</front><back>
<ref-list>
<ref id="B1">
<label>1</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[HORI]]></surname>
<given-names><![CDATA[K.]]></given-names>
</name>
<name>
<surname><![CDATA[MARSUDI]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<name>
<surname><![CDATA[UNNO]]></surname>
<given-names><![CDATA[H.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Simultaneous production of polyhydroxyalkanoates and rhamnolipids by Pseudomonas aeruginosa]]></article-title>
<source><![CDATA[Biotechnol.. Bioengin]]></source>
<year>2002</year>
<numero>78</numero>
<issue>78</issue>
<page-range>699-707</page-range></nlm-citation>
</ref>
<ref id="B2">
<label>2</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[CIRSTEA]]></surname>
<given-names><![CDATA[D.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Use of some carbon sources by Pseudomonas strains for synthesizing polyhydroxyalkanoates and/or rhamnolipids]]></article-title>
<source><![CDATA[Romanian Biotechnological Letters]]></source>
<year>2014</year>
<volume>19</volume>
<numero>3</numero>
<issue>3</issue>
<page-range>9400-8</page-range></nlm-citation>
</ref>
<ref id="B3">
<label>3</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[RASHID]]></surname>
<given-names><![CDATA[N.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Dual-production of polyhydroxyalkanoate and rhamnolipid by Pseudomonas aeruginosa UMTKB-5 using industrial by-products]]></article-title>
<source><![CDATA[Malaysian J. Anal. Sci]]></source>
<year>2021</year>
<numero>25</numero>
<issue>25</issue>
<page-range>24-39</page-range></nlm-citation>
</ref>
<ref id="B4">
<label>4</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[NITSCHKE]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[COSTA]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<name>
<surname><![CDATA[CONTIERO]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Rhamnolipids and PHAs: Recent reports on Pseudomonas-derived molecules of increasing industrial interest]]></article-title>
<source><![CDATA[Process Biochemistry]]></source>
<year>2011</year>
<volume>46</volume>
<numero>3</numero>
<issue>3</issue>
<page-range>621-30</page-range></nlm-citation>
</ref>
<ref id="B5">
<label>5</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[ABDELMONEIM]]></surname>
<given-names><![CDATA[H.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Rhamnolipids: Preparation, Determination and Applications A Review]]></article-title>
<source><![CDATA[American Journal of Food Science and Nutrition Research]]></source>
<year>2017</year>
<volume>4</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>9-17</page-range></nlm-citation>
</ref>
<ref id="B6">
<label>6</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[TORTAJADA]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[FERREIRA DA SILVA]]></surname>
<given-names><![CDATA[L.]]></given-names>
</name>
<name>
<surname><![CDATA[PRIETO]]></surname>
<given-names><![CDATA[M. A.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Second-generation functionalized medium-chain-length polyhydroxyalkanoates: the gateway to high-value bioplastic applications]]></article-title>
<source><![CDATA[International Microbiology]]></source>
<year>2013</year>
<volume>16</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>1-15</page-range></nlm-citation>
</ref>
<ref id="B7">
<label>7</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[ANJUM]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Microbial production of polyhydroxyalkanoates (PHAs) and its copolymers: A review of recent advancements]]></article-title>
<source><![CDATA[International Journal of Biological Macromolecules]]></source>
<year>2016</year>
<numero>89</numero>
<issue>89</issue>
<page-range>161-74</page-range></nlm-citation>
</ref>
<ref id="B8">
<label>8</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[MOZEJKO]]></surname>
<given-names><![CDATA[CIESIELSKA]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Medium-chain-length polyhydroxyalkanoates synthesis by Pseudomonas putida KT2440 relA/spot mutant: bioprocess characterization and transcriptome analysis]]></article-title>
<source><![CDATA[AMB Express]]></source>
<year>2017</year>
<numero>7</numero>
<issue>7</issue>
<page-range>92</page-range></nlm-citation>
</ref>
<ref id="B9">
<label>9</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[MANAVITEHRANI]]></surname>
<given-names><![CDATA[I.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Biomedical Applications of Biodegradable Polyesters]]></article-title>
<source><![CDATA[Polymers]]></source>
<year>2016</year>
<numero>8</numero>
<issue>8</issue>
<page-range>20</page-range></nlm-citation>
</ref>
<ref id="B10">
<label>10</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[PANTAZAKI]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
<name>
<surname><![CDATA[PAPANEOPHYTOU]]></surname>
<given-names><![CDATA[CH.]]></given-names>
</name>
<name>
<surname><![CDATA[LAMBROPOULOU]]></surname>
<given-names><![CDATA[D.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Simultaneous polyhydroxyalkanoates and rhamnolipids production by Thermus thermophiles HB8]]></article-title>
<source><![CDATA[AMB Express]]></source>
<year>2011</year>
<volume>1</volume>
<page-range>17</page-range></nlm-citation>
</ref>
<ref id="B11">
<label>11</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[NOOR]]></surname>
<given-names><![CDATA[FAZIELAWANIE]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Dual-production of polyhydroxyalkanoate and rhamnolipid by Pseudomonas aeruginosa UMTKB-5 USING INDUSTRIAL BY-PRODUCTS]]></article-title>
<source><![CDATA[Malaysian Journal of Analytical Sciences]]></source>
<year>2021</year>
<volume>25</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>24-39</page-range></nlm-citation>
</ref>
<ref id="B12">
<label>12</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[IDOMEH]]></surname>
<given-names><![CDATA[J. E. Co.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Production of Polyhydroxyalkanoates and Biosurfactants using Pseudomonas luteola]]></article-title>
<source><![CDATA[Nigerian Journal of Basic and Applied Science]]></source>
<year>2018</year>
<volume>26</volume>
<numero>2</numero>
<issue>2</issue>
<page-range>67-75</page-range></nlm-citation>
</ref>
<ref id="B13">
<label>13</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[SAHARAN]]></surname>
<given-names><![CDATA[B.]]></given-names>
</name>
<name>
<surname><![CDATA[GREWAL]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
<name>
<surname><![CDATA[KUMAR]]></surname>
<given-names><![CDATA[P.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Biotechnological Production of Polyhydroxyalkanoates: A Review on Trends and Latest Developments]]></article-title>
<source><![CDATA[Chinese Journal of Biology]]></source>
<year>2014</year>
<numero>3</numero>
<issue>3</issue>
<page-range>1-18</page-range></nlm-citation>
</ref>
<ref id="B14">
<label>14</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[RODRÍGUEZ]]></surname>
<given-names><![CDATA[O.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Screening and Characterization of Biosurfactant-Producing Bacteria Isolated from Contaminated Soils with Oily Wastes]]></article-title>
<source><![CDATA[J. Environ. Treat. Tech]]></source>
<year>2017</year>
<volume>5</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>5-11</page-range></nlm-citation>
</ref>
<ref id="B15">
<label>15</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[RAMSAY]]></surname>
<given-names><![CDATA[B.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Production of poly-&#946;-hydroxybutyric-&#946;-hydroxyvaleric acids]]></article-title>
<source><![CDATA[Appl. Environ. Microbiol]]></source>
<year>1990</year>
<numero>56</numero>
<issue>56</issue>
<page-range>2093-8</page-range></nlm-citation>
</ref>
<ref id="B16">
<label>16</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[VALENTIN]]></surname>
<given-names><![CDATA[H.]]></given-names>
</name>
<name>
<surname><![CDATA[REISER]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<name>
<surname><![CDATA[GRUYS]]></surname>
<given-names><![CDATA[K]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) formation from &#947;-aminobutyrate and glutamate]]></article-title>
<source><![CDATA[Biotechnology and Biongineering.]]></source>
<year>2000</year>
<volume>67</volume>
<numero>3</numero>
<issue>3</issue>
<page-range>291-9</page-range></nlm-citation>
</ref>
<ref id="B17">
<label>17</label><nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[AIBA]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<name>
<surname><![CDATA[HUMPHREY]]></surname>
<given-names><![CDATA[A. E.]]></given-names>
</name>
<name>
<surname><![CDATA[MILLIS]]></surname>
<given-names><![CDATA[N. F.]]></given-names>
</name>
</person-group>
<source><![CDATA[Biochemical engineering]]></source>
<year>1973</year>
<edition>2</edition>
<publisher-loc><![CDATA[New York ]]></publisher-loc>
<publisher-name><![CDATA[Academic Press]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B18">
<label>18</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[RIIS]]></surname>
<given-names><![CDATA[V.]]></given-names>
</name>
<name>
<surname><![CDATA[MAI]]></surname>
<given-names><![CDATA[W.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Gas chromatography determination of poly-bhydroxybutyric acid in microbial biomass-esther hydrochloric acid propanolisis]]></article-title>
<source><![CDATA[J. Chromatogr]]></source>
<year>1988</year>
<numero>445</numero>
<issue>445</issue>
<page-range>285-9</page-range></nlm-citation>
</ref>
<ref id="B19">
<label>19</label><nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[LOPES]]></surname>
<given-names><![CDATA[A. K.]]></given-names>
</name>
</person-group>
<source><![CDATA[Produção de ramnolipídios por isolados de Pseudomonas: avaliação do efeito das fontes de carbono e nitrogênio na composição do ramnolípidio]]></source>
<year>2011</year>
<publisher-loc><![CDATA[São Paulo ]]></publisher-loc>
<publisher-name><![CDATA[Instituto de Ciências Biomédicas, Universidade de São Paulo]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B20">
<label>20</label><nlm-citation citation-type="">
<source><![CDATA[STATGRAPHICS CENTURION, X.V.I. Statpoint Technologies. INC. version, 15.2, 17]]></source>
<year>2009</year>
</nlm-citation>
</ref>
<ref id="B21">
<label>21</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[MARSUDI]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<name>
<surname><![CDATA[UNNO]]></surname>
<given-names><![CDATA[H.]]></given-names>
</name>
<name>
<surname><![CDATA[HORI]]></surname>
<given-names><![CDATA[K.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Palm oil utilization for the simultaneous production of polyhydroxyalkanoates and rhamnolipids by Pseudomonas aeruginosa]]></article-title>
<source><![CDATA[Appl Microbiol Biotechnol]]></source>
<year>2008</year>
<numero>78</numero>
<issue>78</issue>
<page-range>955-61</page-range></nlm-citation>
</ref>
<ref id="B22">
<label>22</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[COSTA]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Cassava wastewater as a substrate for the simultaneous production of rhamnolipids and polyhydroxyalkanoates by Pseudomonas aeruginosa]]></article-title>
<source><![CDATA[Journal of Industrial Microbiology &amp; Biotechnology]]></source>
<year>2009</year>
<volume>36</volume>
<numero>8</numero>
<issue>8</issue>
<page-range>1063-72</page-range></nlm-citation>
</ref>
<ref id="B23">
<label>23</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[CHEN]]></surname>
<given-names><![CDATA[F.]]></given-names>
</name>
<name>
<surname><![CDATA[CHEN]]></surname>
<given-names><![CDATA[CH.]]></given-names>
</name>
<name>
<surname><![CDATA[RIADI]]></surname>
<given-names><![CDATA[L.]]></given-names>
</name>
<name>
<surname><![CDATA[JU]]></surname>
<given-names><![CDATA[L.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Modeling RHL Quorum-Sensing Regulation on Rhamnolipid Production by Pseudomonas aeruginosa]]></article-title>
<source><![CDATA[Biotechnol. Prog]]></source>
<year>2004</year>
<numero>20</numero>
<issue>20</issue>
<page-range>1325-31</page-range></nlm-citation>
</ref>
<ref id="B24">
<label>24</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[DE LIMA]]></surname>
<given-names><![CDATA[C. J]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Biosurfactant Production by Pseudomonas aeruginosa Grown in Residual Soybean Oil.]]></article-title>
<source><![CDATA[Appl. Biochem. Biotechnol]]></source>
<year>2009</year>
<numero>152</numero>
<issue>152</issue>
<page-range>156-68</page-range></nlm-citation>
</ref>
<ref id="B25">
<label>25</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[PERFUMO]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Rhamnolipids are conserved biosurfactants molecules: implications for their biotechnological potential]]></article-title>
<source><![CDATA[Appl. Microbiol. Biotechnol]]></source>
<year>2013</year>
<volume>97</volume>
<numero>16</numero>
<issue>16</issue>
<page-range>7297-306</page-range></nlm-citation>
</ref>
</ref-list>
</back>
</article>
