<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>2224-5421</journal-id>
<journal-title><![CDATA[Revista Cubana de Química]]></journal-title>
<abbrev-journal-title><![CDATA[Rev Cub Quim]]></abbrev-journal-title>
<issn>2224-5421</issn>
<publisher>
<publisher-name><![CDATA[Ediciones UO, Universidad de Oriente]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S2224-54212023000200185</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Inserciones conservadas en secuencias de proteínas para estudios moleculares en el género Rhodomicrobium]]></article-title>
<article-title xml:lang="en"><![CDATA[Conserved insertions in protein sequences for molecular studies in the genus Rhodomicrobium]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Cutiño-Jiménez]]></surname>
<given-names><![CDATA[Ania Margarita]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[González-Vicente]]></surname>
<given-names><![CDATA[Andy Manuel]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad de Oriente Centro de Estudios de Biotecnología Industrial (CEBI) ]]></institution>
<addr-line><![CDATA[Santiago de Cuba ]]></addr-line>
<country>Cuba</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Hospital Provincial Clínico Quirúrgico Docente &#8220;Saturnino Lora Torres&#8221;  ]]></institution>
<addr-line><![CDATA[Santiago de Cuba ]]></addr-line>
<country>Cuba</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>08</month>
<year>2023</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>08</month>
<year>2023</year>
</pub-date>
<volume>35</volume>
<numero>2</numero>
<fpage>185</fpage>
<lpage>199</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_arttext&amp;pid=S2224-54212023000200185&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_abstract&amp;pid=S2224-54212023000200185&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_pdf&amp;pid=S2224-54212023000200185&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[RESUMEN El género Rhodomicrobium comprende especies importantes para la agricultura y el medio ambiente. Sin embargo, pocas investigaciones se han dirigido a la identificación de marcadores moleculares que distingan a sus miembros de otros grupos de bacterias. Los marcadores Indeles (inserciones y deleciones) en secuencias de proteínas son útiles para estudios evolutivos y taxonómicos en bacterias. Se analizaron secuencias homólogas de las proteínas ADN ligasa NAD+ dependiente, y Serina ARNt sintetasa, obtenidas de la base de datos UniprotKB/Swiss-Prot, y posteriormente alineadas con el programa MUSCLE. El análisis filogenómico se realizó por el método de Máxima Verosimilitud con el programa RAxML. Se identificaron inserciones que soportan la monofilia del género y confirman que Rhodomicrobium lacus es un grupo hermano de R. vannielii y R. udaipurense. Las inserciones analizadas constituyen marcadores moleculares importantes para estudios taxonómicos y evolutivos en el género Rhodomicrobium, y para futuros estudios bioquímicos o funcionales en dichas enzimas.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[ABSTRACT The genus Rhodomicrobium comprises species with agricultural and environmental importance. However, few investigations have been conducted to identify molecular markers that could be used to distinguish members of Rhodomicrobium from other groups of bacteria. Conserved signature indels (insertions and deletions) in protein sequences are useful for evolutionary and taxonomic studies in bacteria. Homologous sequences of the proteins NAD+ dependent DNA ligase and Serine-tRNA synthetase were obtained from the UniprotKB/Swiss-Prot database and aligned using MUSCLE programme. Phylogenomic analysis was performed through the Maximum Likelihood method with RAxML. Insertions were identified which support the monophyly of this genus and confirm that Rhodomicrobium lacus forms a sister group of R. vannielii and R. udaipurense. Thus, the insertions analyzed constitute important molecular markers for taxonomic and evolutionary studies in Rhodomicrobium, and also for future biochemical or functional studies in those enzymes.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Rhodomicrobium]]></kwd>
<kwd lng="es"><![CDATA[identificación]]></kwd>
<kwd lng="es"><![CDATA[marcadores moleculares]]></kwd>
<kwd lng="es"><![CDATA[indeles.]]></kwd>
<kwd lng="en"><![CDATA[Rhodomicrobium]]></kwd>
<kwd lng="en"><![CDATA[identification]]></kwd>
<kwd lng="en"><![CDATA[molecular markers]]></kwd>
<kwd lng="en"><![CDATA[indels.]]></kwd>
</kwd-group>
</article-meta>
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