<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>2224-6185</journal-id>
<journal-title><![CDATA[Tecnología Química]]></journal-title>
<abbrev-journal-title><![CDATA[RTQ]]></abbrev-journal-title>
<issn>2224-6185</issn>
<publisher>
<publisher-name><![CDATA[Universidad de Oriente]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S2224-61852019000100001</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Optimización del medio de cultivo para la producción de biomasa y compuestos fenólicos por Pleurotus ostreatus en fase sumergida utilizando la metodología de superficie de respuesta]]></article-title>
<article-title xml:lang="en"><![CDATA[Optimization of medium composition for the production of Pleurotus ostreatus biomass and phenols in submerged fermentation with response surface methodology]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Ferrer-Romero]]></surname>
<given-names><![CDATA[Juan Carlos]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Mas-Diego]]></surname>
<given-names><![CDATA[Siannah María]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Beltrán-Delgado]]></surname>
<given-names><![CDATA[Yaixa]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Rodríguez-Quiala]]></surname>
<given-names><![CDATA[Yunior]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Morris Quevedo]]></surname>
<given-names><![CDATA[Humberto Joaquín]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad de Oriente Facultad de Ingeniería Química y Agronomía ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Cuba</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Universidad de Oriente Centro Nacional de Electromagnetismo Aplicado ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Cuba</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,Universidad de Oriente Centro de Estudios de Biotecnología Industrial ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Cuba</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>04</month>
<year>2019</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>04</month>
<year>2019</year>
</pub-date>
<volume>39</volume>
<numero>1</numero>
<fpage>1</fpage>
<lpage>16</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_arttext&amp;pid=S2224-61852019000100001&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_abstract&amp;pid=S2224-61852019000100001&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_pdf&amp;pid=S2224-61852019000100001&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[RESUMEN Pleurotus ostreatus es una de las especies de hongos comestibles más extensamente producida en el mundo, por la calidad nutricional de su biomasa y la presencia de metabolitos de importancia farmacológica. Por ello, en la actualidad existe un marcado interés en la obtención de biomasa micelial y compuestos bioactivos de manera más rápida y eficiente. En el presente trabajo se utilizó la metodología de superficie de respuesta en la optimización del medio de cultivo YPG, con el objetivo de obtener la máxima concentración de biomasa y fenoles totales posible, mediante fermentación sumergida de Pleurotus ostreatus. Partiendo de un diseño compuesto central, se ajustaron dos modelos matemáticos polinomiales de segundo orden a los resultados experimentales obtenidos de la producción de biomasa micelial y fenoles totales. La concentración de nitrógeno tuvo una mayor influencia significativa en la producción de biomasa. Tanto las concentraciones de nitrógeno como la de carbono influyeron significativamente sobre la concentración de fenoles totales. Los modelos predijeron un valor máximo de concentración de biomasa igual a 15,28 g·L-1, para un medio de cultivo con 28,41 g·L-1 de glucosa, 5,68 g·L-1 de extracto de levadura y 2,32 g·L-1 peptona; así como un valor máximo de concentración de fenoles totales igual a 104 µg·mL-1 para la siguiente composición del medio de cultivo:11,59 g·L-1 de glucosa, 2,32 g·L-1 de extracto de levadura y 2,32 g·L-1 de peptona.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[ABSTRACT There is a marked interest in obtaining mycelial biomass and bioactive compounds more quickly and efficiently, from edible fungi. Pleurotus ostreatus is one of the most widely cultivated species in the world, due to the nutritional quality of its biomass and the presence of metabolites of pharmacological importance. In the present work, the response surface methodology was used in the optimization of the YPG culture medium, aiming to obtain the maximum concentration of biomass and total phenols, by means of submerged fermentation of Pleurotus ostreatus. Based on a central composite design, two second-order polynomial mathematical models were fitted to the experimental results obtained from the production of mycelial biomass and total phenols. It was observed that the concentrations of the nitrogen sources had bigger significant influence on the production of biomass. Both the nitrogen and carbon source concentrations had a significant influence on the total phenol concentration. The models predicted a maximum biomass concentration value of 15,28 g·L-1 for a culture medium with 28,41 g·L-1 glucose, 5,68 g·L-1 yeast extract and 2,32 g·L-1 peptone, as well as a maximum total phenols concentration of 104 &#956;g·mL-1 for the following culture medium composition: 11,59 g·L-1 glucose, 2,32 g·L-1 yeast extract and 2,32 g·L-1 peptone.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Optimización]]></kwd>
<kwd lng="es"><![CDATA[metodología de superficie de respuesta]]></kwd>
<kwd lng="es"><![CDATA[Pleurotus ostreatus]]></kwd>
<kwd lng="es"><![CDATA[fermentación sumergida]]></kwd>
<kwd lng="es"><![CDATA[fenoles]]></kwd>
<kwd lng="en"><![CDATA[Optimization]]></kwd>
<kwd lng="en"><![CDATA[response surface methodology]]></kwd>
<kwd lng="en"><![CDATA[Pleurotus ostreatus]]></kwd>
<kwd lng="en"><![CDATA[submerged fermentation]]></kwd>
<kwd lng="en"><![CDATA[phenols]]></kwd>
</kwd-group>
</article-meta>
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