<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>2224-6185</journal-id>
<journal-title><![CDATA[Tecnología Química]]></journal-title>
<abbrev-journal-title><![CDATA[RTQ]]></abbrev-journal-title>
<issn>2224-6185</issn>
<publisher>
<publisher-name><![CDATA[Universidad de Oriente]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S2224-61852019000100117</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Estudio de nuevas alternativas para obtener altas concentraciones de proteasas alcalinas a partir del hongo Lecanicillum sp]]></article-title>
<article-title xml:lang="en"><![CDATA[Study of new alternatives to obtain of high concentrations of alkaline proteases from fungus Lecanicillum sp]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[San Juan Rodríguez]]></surname>
<given-names><![CDATA[Ana Nelis]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Gómez Santiesteban]]></surname>
<given-names><![CDATA[Eulalia]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Guevara Verdecia]]></surname>
<given-names><![CDATA[Yusmila]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Dopico Ramirez]]></surname>
<given-names><![CDATA[Daisy]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,, Instituto Cubano de Investigaciones de los Derivados de la Caña de Azúcar UEB Bioprocesos Cuba 10 ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Cuba</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>04</month>
<year>2019</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>04</month>
<year>2019</year>
</pub-date>
<volume>39</volume>
<numero>1</numero>
<fpage>117</fpage>
<lpage>134</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_arttext&amp;pid=S2224-61852019000100117&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_abstract&amp;pid=S2224-61852019000100117&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.sld.cu/scielo.php?script=sci_pdf&amp;pid=S2224-61852019000100117&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[RESUMEN El desarrollo de procesos biotecnológicos ha permitido obtener productos eficientes y rentables para diferentes aplicaciones en la agricultura, entre ellas el control biológico de plagas. En el presente trabajo se evaluaron diferentes alternativas para incrementar la producción de proteasas alcalinas extracelulares, por el hongo Lecanicillium sp. cepa 3166, las que presentan una elevada actividad nematicida. Se evaluó el crecimiento del hongo y la producción de proteasas alcalinas a diferentes valores de pH inicial, demostrándose que el pH es un factor influyente en la inducción de las proteasas alcalinas. Con el objetivo de formular un medio de cultivo se desarrolló un plan factorial 23, lo que evidenció que los factores de mayor significación sobre la producción de proteasas alcalinas fueron la relación C/N y la fuente de nitrógeno, que se favoreció con el uso de la relación C/N=1 y la harina de soya. En las experiencias realizadas en modo discontinuo con incrementos de nutrientes hasta fermentador de 10 litros, se favoreció la producción de proteasas alcalinas y se alcanzó una actividad proteolítica máxima de 0,35 mg mL-1 y 0,52 mg mL-1 respectivamente.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[ABSTRACT Biotechnological process development has made possible the obtaining of efficient and profitable products for different applications in agriculture, including biologic control of pests. In the present work, different choices were evaluated in order to increase extracellular alkaline proteases production for the fungus Lecanicillium sp. strain 3166. These enzymes showed high nematicide activity. The growing rate of microorganism and the enzyme production were evaluated at different starting pH, which proved to have influence on alkaline proteases induction. A 23 factorial design was made in order to formulate a culture media, being C/N relation and nitrogen source the most significant factors. The relation C/N=1 and soja bean flour were the most favoured. Experiences in 10 liters fermenter using batch and fed-batch culture showed an improvement in alkaline proteases production. The maxime proteolitic activities were 0,35 mg mL-1 y 0,52 mg mL-1 respectively.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[proteasas alcalinas]]></kwd>
<kwd lng="es"><![CDATA[Lecanicillium spp]]></kwd>
<kwd lng="es"><![CDATA[control biológico]]></kwd>
<kwd lng="es"><![CDATA[Meloidogyne incognita]]></kwd>
<kwd lng="en"><![CDATA[alkaline proteases]]></kwd>
<kwd lng="en"><![CDATA[Lecanicillium sp]]></kwd>
<kwd lng="en"><![CDATA[biologic control]]></kwd>
<kwd lng="en"><![CDATA[Meloidogyne incognita]]></kwd>
</kwd-group>
</article-meta>
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